Fig 2: HuR binds to the 3′UTR of FOXM1 and regulates its transcription. a and b Pearson's correlation scatter plots for FOXM1-SOCS7 (a) and FOXM1-HuR (b) of HGSOC patients (n = 30) in cohort 1. c Additional HuR RIPs followed by qRT-PCR on precipitates validated HuR binding of FOXM1 mRNA in CAOV3 cells transduced with indicated lentiviral vectors. d Relative luciferase activity of FOXM1 3'UTR in CAOV3 cells transduced with indicated lentiviral vectors. e Relative mRNA levels of FOXM1 in CAOV3 cells transduced with indicated lentiviral vectors and treated with 5 μg/ml actinomycin D. f-i HuR and FOXM1 expression in CAOV3 cells transduced with indicated lentiviral vectors. *P < 0.05, **P < 0.01 and ***P < 0.001, in related with shNC, Vector. ###P < 0.001, in related with SOCS7

Fig 3: HuR is involved in the regulation of HGSOC cell viability and cell cycle progression by SOCS7. a HuR overexpression in CAOV3 cells. b Viability, c and d cell cycle distribution of CAOV3 cells transduced with indicated lentiviral vectors. e HuR protein expression in CAOV3 cells transduced with indicated lentiviral vectors. **P < 0.01, ***P < 0.001, in related with Vector. ###P < 0.001, in related with SOCS7

Fig 4: Interactions between HuR and SOCS7. a Purification of SOCS7 immunocomplex (arrows). Proteins were separated on SDS-PAGE and stained by Coomassie Blue. b and c The interactions between HuR and SOCS7 in CAOV3 (b) and OVCAR3 (c) cell lysates. d Subcellular localizations of SOCS7 (green) and HuR (red). Nuclei were stained with DAPI (blue) for reference. Scale bar = 50 μm. e and f The mRNA (e) and protein (f) levels of HuR in CAOV3, OVCAR3, and COV318 cells transduced with indicated lentiviral vectors. g SOCS7 and HuR protein expression in CAOV3 and OVCAR3 cells transduced with indicated lentiviral vectors and treated with 10 μM proteasome inhibitor (MG132) or DMSO. h SOCS7/HuR protein expression and the ubiquitination of HuR in CAOV3 cells