Fig 1: GOT1 regulates adipose cell differentiation by altering the NADPH content. (A, C) mRNA expression levels of GOT1, GOT2, MDH1, and ME1 in the 3T3-L1 cells after transfection of knockout (A) and overexpression plasmids (C). (B, D) protein levels of GOT1 and MDH1 in the 3T3-L1 cells after transfection of knockout (B) and overexpression plasmids (D). (E, F) NADPH content in the 3T3-L1 cells after transfection of knockout (E) and overexpression plasmids (F). The results are shown as NADP+/NADPH ratios. Data are shown as means±standard error of the mean. GOT1, glutamic-oxaloacetic transaminase 1; NADPH, nicotinamide adenine dinucleotide phosphate; MDH1, malate dehydrogenase 1; ME1, malic enzyme. * Indicate significant difference from the control at p<0.05 and ** p<0.01, respectively.