Fig 2: CT suppressed the protein expression of TRPM2 and NHE1 in carotid arteries of T2DM rats. (A) The immunofluorescence detection of TRPM2 in carotid arteries (TRPM2, green; a-SMA, red). (B) Quantitative analysis of fluorescence intensity of TRPM2. (C) The immunohistochemistry detection of TRPM2 in carotid arteries. (D) Quantitative analysis of positive intensity of TRPM2. (E) The immunofluorescence detection of NHE1 in carotid arteries (NHE1, green; a-SMA, red). (F) Quantitative analysis of fluorescence intensity of NHE1. (G) The immunohistochemistry detection of NHE1 in carotid arteries. (H) Quantitative analysis of positive intensity of NHE1 (N = 6). (I,J) Western blot analysis of TRPM2 and NHE1 in carotid arteries (N = 3). Data were analyzed by one-way ANOVA by Tukey post hoc tests or Bonferroni post hoc analyses. All data were expressed as mean ± SEM. # p < 0.05 vs. Control group, * p < 0.05 vs. T2DM group.

Fig 3: CT had no effect on the expression of NHE1 protein in TRPM2 knockout mice. (A) The immunofluorescence detection of NHE1 in carotid arteries (NHE1, green; a-SMA, red). (B) Quantitative analysis of fluorescence intensity of NHE1. (C) The immunohistochemistry detection of NHE1 in carotid arteries. (D) Quantitative analysis of positive intensity of NHE1. (E) Detection of mitochondrial membrane potential in carotid arteries (JC-1aggregates, red; JC-1 monomers, green; a-SMA, purple). (F) The quantitative analysis of the mitochondrial membrane potential (??m). (G–J) Western blot analysis of NHE1, NOX2, NOX4, and DRP1 in carotid arteries (N = 3). Data were analyzed by one-way ANOVA by Tukey post hoc tests or Bonferroni post hoc analyses. All data were expressed as mean ± SEM. # p < 0.05 vs. TRPM2+/+ group.