beta Actin Polyclonal Antibody bs-0061R from Bioss Inc.

Product Specs
Itembeta Actin Polyclonal Antibody
CompanyBioss Inc.
Price $99.00Supplier Page View Company Product Page
Catalog Numberbs-0061R
Molecular Weight42kDa
ImmunogenMAP conjugated synthetic peptide derived from human beta Actin
ApplicationsIF(IHC-F), WB, WB, FCM, WB, IHC-P, IHC, WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC), WB, IHC, FCM, WB, Others, WB, IF, IHC-P, ELISA
ReactivitySheep, Monkey, Bacteria, Mouse, Zebrafish, Bovine, Goat, Bovine, Fish, Rabbit, Others, Human, Rat, Chicken, Human, Dog, Other Species, Human, Mouse, Rat, Hamster, All, Human, Mouse, Fish, Pig, Rat, Human, Chicken, Mouse
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
Quantity100ul
Conjugate/TagUnconjugated
IsotypeIgG
ClonalityPolyclonal
Antibody TypePrimary Antibody
HostRabbit
Targetbeta-Actin
Concentration1ug/ul
Tissueheart, brain tissue (Medulla oblongata), PC12, cardiac tissues, brain, colon, spinal cord, villus (intestinal wall), Cerebral cortex, kidney, testis, Stomach, knee joint samples, Corneas, synovial tissue, zebrafish, Lung, cerebral cortex, mammary gland, muslce, testis, tumor tissue, liver, hepatocytes, gastric tissue, nodular goiter, testicular samples, placenta, cardiac muscle, PC12 cells, tumor, Brain, human peritoneal tissues, cartilage, uterus, bursa of Fabriciu
NCBI Full Gene Nameactin beta
NCBI Gene AliasesPS1TP5BP1, BRWS1

Questions and Answers

Can this beta Actin antibody be used in Western blotting for detecting protein expression in various species?

Yes, the beta Actin Polyclonal Antibody has been shown to work effectively in Western blotting applications across multiple species, including human, mouse, rat, and others, as indicated by its use in various scientific studies.

Is this antibody suitable for immunofluorescence applications, and what has been reported in the literature?

The beta Actin Polyclonal Antibody is suitable for immunofluorescence applications. It has been utilized in studies to visualize actin filaments in different cell types, demonstrating its effectiveness in this application as referenced in multiple publications.

What specific tissues have been analyzed using this antibody in research?

This antibody has been used to analyze a variety of tissues, including kidney, liver, lung, brain, and heart, among others, as evidenced by its application in numerous studies focusing on cellular structures and functions.

How has this antibody been utilized in studies related to cellular stress responses?

The beta Actin Polyclonal Antibody has been employed in studies examining cellular stress responses, such as those involving oxidative stress and heat shock, where it serves as a loading control to ensure accurate quantification of protein levels in Western blot analyses.

Bioss Inc.

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Woburn, MA 01801

United States

Phone: 800.501.7654 (Toll free)
Phone: +1.781.569.5821 (International)

Email: info@biossusa.com
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Company Profile

Website: www.biossusa.com

(1) Delivery of miR-25802 via Small Vesicles Protects Against Mitochondrial Injury, Oxidative Stress, and Neuroinflammation in Alzheimer's DiseaseMol NeurobiolApril 23, 2026Hamit Çelik, Elif Dalkılınç, Şeyma Aydın, Oğuz Çelik, Sefa Küçükler, Ahmet Topal, Ramazan Akay, Sinan Gönüllü, Mustafa Onur Yıldız, Bülent Alım, et al.with primary antibodies against BDNF (Cat. No.: ab108319, 1:1000, Abcam, UK) and β-actin (Cat. No.: bs-0061R, 1:1000, Bioss, USA). After washing, the membranes were incubated at room temperature for 1 h with HRP-conjugated

(2) MAGI3 deficiency unleashes β-catenin conformational change to drive metastatic progression and mTOR inhibitor resistance in ccRCCCell Death & DiseaseMarch 24, 2026Siyu Gu, Haibo Wang, Hua Liu, Yumeng Yang, Yu Guo, Pengyan Fa, Lijie Zhang, Yang Yang, Xuan Qi, Qiong Qin, et al.Cell Signaling Technology) and phospho-specific (Ser33/37/Thr41; #9561, Cell Signaling Technology), β-actin: bs-0061R (Bioss), used as the loading control. GST pull-down assay We performed GST pull-down assays using

(3) NUAK1 silencing enhances radiotherapy-induced ferroptosis in locally advanced rectal cancer by impairing Nrf2-driven transcription of GPX4Cell Oncol (Dordr)March 18, 2026Zhicheng Zhuang, Bingjie Guan, Ye Wang, Xinru Lin, Huajun Cai, Bin Chen, Shoufeng Li, Xing Liu, Jinfu Zhuang, Guoxian Guan°C with primary antibodies against NUAK1 (22723-1-AP, Proteintech), GPX4 (ab125066, Abcam), β-actin (bs-0061R, Bioss), Nrf2 (16396-1-AP, Proteintech), Lamin B1 (12987-1-AP, Proteintech), MYPT1 (22117-1-AP, Proteintech),

(4) Exosomal lncRNA OTUD6B-AS1 as a pathogenic nanocarrier promotes inflammatory macrophage polarization in endometritis via a targetable ceRNA circuitMater Today BioMarch 14, 2026Jing Yang, Yajing Chen, Kui Wang, Lifang Mi, Yushan Chen, Xiaobing Li, Gefen Yin, Kangfeng Jiang(Santa Cruz Biotechnology, Cat#sc-51869), RBP-Jκ (Santa Cruz Biotechnology, Cat#sc-271128), and β-actin (Bioss, Cat#bs-0061R). 2.16 Statistical analysis All quantitative data are expressed as the mean ± standard error of the

(5) Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial AutophagyCell Mol NeurobiolMarch 6, 2026Pengyu Duan, Yonghong Bi, Weiyu Feng, Zhehao Jin, Yao Meng, Hangbing Li, Xiaoyan Li, Lan Luo, Xiangcheng Zhao, Bing Zhangdetected using enhanced chemiluminescence kits (P10300, New Cell & Molecular Biotech). β-actin (1:2 000, bs-0061R, AB_10855480, Bioss) and Histone H3 (1:2 000, bs-0349R, RRID:AB_10857220, Bioss) was used as the protein

(6) Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signallingChin MedMarch 6, 2026Zeyu Zhang, Zhihua Yang, Zhuangzhuang Jia, Yuwei Song, Liuli Guo, Shuai Wang, Xianliang Wang, Jingyuan Maop-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang,

(7) Electroacupuncture Inhibits the Early Neuroinflammatory Cascade Triggered by TLR2 in the Prodromal Period of PDJ Inflamm ResMarch 5, 2026Ke Xu, Yuan Li, Bing-Cheng Hu, Yu Zhang, Yan Bai, Shun WangTechnology Co., Ltd., Beijing) to reduce nonspecific binding. After blocking, the primary antibody TH (#bs-0061r, Beijing Bioss Biotechnology Co., Ltd., dilution ratio 1:300) was applied, and the sections were incubated

(8) Exosomes derived from bone marrow mesenchymal stem cells facilitate repair of radiation-Induced skin injury by attenuating inflammation and apoptosisScientific ReportsFebruary 2, 2026Yuxin Wen, Yiwei Song, Shichao Pan, Na Zhang, Xu Tongfollowed by overnight incubation at 4 °C with the following primary antibodies: β-actin (1:6000, Bioss, bs-0061R, Beijing, China), Arg-1 (1:1000, Wanlei, WL02825, Shenyang, China), iNOS (1:1000, Wanlei, WL0992a, Shenyang,

(9) DNA damage-inducible transcript 3-mediated endoplasmic reticulum stress drives manganese-induced apoptosis in bovine testicular Leydig cellsCell Stress and ChaperonesFebruary 1, 2026Liwei Huang, Yu Cheng, Xiaolong Pan, Hongxia Li, Jia Liu, Guoqing Zhao, Lijuan Huang, Lili Wang, Chunying Song, Pengkang Song, et al.The membranes were incubated with primary antibody DDIT3 (15204-1-AP, Proteintech, 1:1000), β-actin (bs-0061R, Bioss, 1:10,000) at 4 °C overnight. Incubate the membrane with the secondary antibody at room temperature

(10) Integrative bioinformatics and experimental analysis reveals FRA1 as a key mediator of tubulointerstitial inflammation in lupus nephritisMolecular Medicine ReportsJanuary 28, 2026Wenpeng Ni, Jialin He, Zhouyu Zeng, Jialong Ke, Runpei Lin, Jianming Peng, Kunyi Deng, Lijuan Wen, Yanhui Chen, Chilun Zhang, et al.Tween-20 (TBST). The primary antibodies used in the present study included: β-actin (1:5,000; cat. no. bs-0061R; BIOSS), FRA1 (1:1,000; cat. no. A5372; ABclonal Biotech Co., Ltd.), IL-1β (1:1,000; cat. no. HA601002;

(11) Efficacy of neonatal mouse muscle extracellular vesicles in skeletal muscle repair and regenerationCell RegenerationJanuary 23, 2026Chengwei Liu, Zhouyan Li, Xinyue Liu, Sitong Lv, Xijun Yinanti-MYOG (bs-1299R), anti-MYHC (bs-9863R), anti-DESMIN (bs-1135R), anti-MF-20 (bs-10985R), anti-ACTIN (bs-0061R), and anti-TUBULIN (bs-4513R) (all from BIOSS, Beijing, China; 1:2000). After washing, membranes were

(12) Alfalfa Polysaccharide Improves Rabbit Growth by Modulating Gut Microbiota and Suppressing Inflammation Through PPARγ/NF-κB PathwayInternational Journal of Molecular SciencesJanuary 19, 2026Junying Xu, Fang Li, Yuanxin Zhu, Dongmei Liu, Wenjing Duan, Ran Yu, Boshuai Liu, Hao Sun, Zhichang Wang, Defeng Li, et al.Health, Bethesda, MD, USA). For rabbit tissue experiments, the primary antibodies were β-actin (BIOSS, bs-0061R, 1:5000), NF-κB p65 (ZNGBIO, 380172, 1:1000), p-NF-κB p65 (CST, 3033, 1:1000), and PPARγ (BIOSS, bs-0530R,

(13) Si-Ni-San improves the deposition of lipid droplets in MAFLD through modulating the FXR-GPAT4 axisChinese MedicineJanuary 13, 2026Haibo Fan, Yalei Hou, Yue Li, Zhiwen Zheng, Xuelun Wang, Yunfeng Li, Yongmin LiChina, 1:1000), ATGL (R389129-50, Bioss, China, 1:1000), LC3Ⅰ/Ⅱ (ABC929, Merck, USA, 1:1000), β-actin (bs-0061R, Bioss, China, 1:5000) and GAPDH (GB11002-100, Servicebio, China, 1:5000). After 12 h of incubation

(14) NCOA7 promotes OSCC progression by inhibiting ROS-regulated ferroptosisCellular and Molecular Life SciencesJanuary 6, 2026Hang Si, Yun Feng, Xiaoyan Zhang, Tiejun Zhou, Xinyue Liao, Yongxian Lai, Yan Feng, Li Yu(Beyotime, P0252, Sichuan, China) for 5 min, followed by incubating with β-actin antibody (1:5000, bs-0061R, Bioss, Beijing, China), SLC7A11 antibody (1:1000, HA600098, HUABIO, Hangzhou, China), GPX4 antibody

(15) Serum AKR1A1 Levels Predict eGFR Decline Rate in Black Americans with Type 2 DiabetesKidney360January 1, 2026Lijun Ma, Young A. Choi, DengFeng Li, Moin A. Saleem, Barry I. Freedman(1:500; Santa Cruz, sc-271723) and rabbit polyclonal anti-human β-actin (1:3000; Bioss Antibodies, bs-0061R). The membrane was then washed three times in Tris-buffered saline containing 0.1% Tween 20 and incubated

(16) The influence of CXCL9 on M2 macrophages in lung cancer developmentTranslational Cancer ResearchDecember 31, 2025Lin Mi, Liyun Xu, Zhijun Chen, Yeye Zheng(bs-0412R, bs-4593R), ERK/p-ERK (bs-1020R, bs2469R), AKT/p-AKT (bs-0115R and bs-0876R), and β-actin (bs-0061R) (Bioss Antibodies, Woburn, MA, USA), along with corresponding horseradish peroxidase (HRP)-conjugated

(17) The novel thioredoxin reductase inhibitor butaselen suppresses lung cancer by inducing oxidative stressRedox ReportDecember 31, 2025Yifan Chen, Guangliang Qiang, Liang Jin, Yan Sun, Guozhou Zhang, Fengling Hu, Jinhui Feng, Feng Wei, Shengxin Zeng, Lixiang Xue, et al.(Proteintech, 24206-1-AP), Bcl-2 (Proteintech, 12789-1-AP), Bax (Proteintech, 50599-2-Ig), β-actin (Bioss, bs-0061R), Flag (Sigma-Aldrich, F1804), HA (Covance, MMS-101P), p53 (Santa, sc-126), p21 (MBL, K0081-3), p27

(18) Notoginsenoside R1 mitigates UVB-induced skin sunburn injury through modulation of N4-acetylcytidine and autophagyChin MedDecember 18, 2025Shuyun Liang, Xiaokang Liu, Yuting Yang, Fangyuan Zhang, Xiaobo Sun, Tong Zhang, Dean Guo, Jiyu Gong, Zizhao Yangfollowing primary antibodies were employed:Anti-GAPDH (Proteintech, Cat#10494, 1:2000),Anti-β-actin (Bioss, bs-0061R, 1:2000),Anti-AKT1 (Servicebio, GB13011, 1:500),Anti-PI3K (Proteintech, Cat#27921, 1:500),Anti-mTOR

(19) Taurine Alleviates Inflammation, Oxidative Stress, Apoptosis, and Uterus Microbiota Dysregulation of Endometritis by Inhibiting PI3K-AKT/MAPK/NF-κB Pathways in MiceAnimals (Basel)December 16, 2025Jianxu Xiao, Chongliang Bi, Ming Yang, Chen Chen, Juanjuan Zhao, Xiaoqing Huang, Jingyuan Zhang, Buwei Yin, Ke Li, Yuzhong MaAntibodies,Manufacturers,Catalog Numbers,Dilution Ratios β-actin,"Bioss (Bioss Inc., Woburn, MA, USA)",bs-0061R,1:5000 p65,Bioss,bs-0465R,1:1000 phospho-p65,Bioss,bs-0982R,1:1000 IκBα,Bioss,bs-1287R,1:1000 phospho-IκBα,Bioss,Bsm-52169R,1:1000 p38,"CST

(20) Quercetin mitigated LPS-induced PANoptosis in the bursa of Fabricius via the cGAS/STING signaling pathway in broilersPoult SciDecember 2, 2025Yiming Zhang, Qihang Jiang, Haodong Hu, Shiping Li, Jiahong Chu, Fuze She, Shu LiRatio cGAS,ABclonal,A8335,1:1000 STING,ABclonal,A3575,1:1000 ZBP1,ABclonal,A13899,1:3000 RIPK1,Wanlei,WL04522,1:500 RIPK3,Lab-made,,1:500 MLKL,Lab-made,,1:500/1:1000 P-MLKL,Bioss,bsm-54104R,1:500 Caspase-8,Wanlei,WL03426,1:1500 Caspase-3,Wanlei,WL02117,1:500/1:1000 C-Caspase-3,Wanlei,WL01992,1:1000 DRP1,Wanlei,WL03028,1:1500 OPA1,Bioss,bsm-54144R,1:500 MFN1,Bioss,bs-0557R,1:1000 MFN2,Bioss,bs-2988R,1:1000 COXI-NDUFB8,ABclonal,A19732,1:1000 COXII-SDHB,ABclonal,A10821,1:1000 COXIII-UQCRC2,ABclonal,A4366,1:1000 COXIV-MTCO1,ABclonal,A17889,1:1000 COV-ATP5A1,ABclonal,A5884,1:1000 NLRP3,Wanlei,WL02635,1:1500 Caspase-1,Wanlei,WL03450,1:1000 GSDMD,Wanlei,WL05686,1:500/1:1000 FADD,Wanlei,WL05489,1:1000 ASC,Wanlei,WL02462,1:1000 IL-1β,Bioss,bs-0812R,1:500 IL-6,Wanlei,WL02841,1:1000 IFN-γ,Wanlei,WL02440,1:1000 TNF-α,Wanlei,WL01581,1:500 β-actin,Bioss,bs-0061R,1:2000 Antibody dilution ratio.

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beta Actin Polyclonal Antibody from Bioss Inc.

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beta Actin Polyclonal Antibody from Bioss Inc.

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