Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 and PE conjugated Rat anti Human TNF alpha (MCA1560PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A for 6 hours were stained with FITC conjugated Mouse anti Human CD3 and PE conjugated Rat IgG1 . Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A for 6 hours were stained with FITC conjugated Mouse anti Human CD3 and PE conjugated Rat anti Human TNF alpha . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat anti Human TNF alpha (MCA1560PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat IgG1 (MCA6004PE). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat anti Human TNF alpha (MCA1560PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat anti Human TNF alpha (MCA1560PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat IgG1 (MCA6004PE). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat anti Human TNF alpha (MCA1560PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.