MOUSE ANTI RAT CD163 MCA342GA from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemMOUSE ANTI RAT CD163
CompanyBio-Rad
Price $326.00Supplier Page View Company Product Page
Catalog NumberMCA342GA
Quantity0.1 mg
ApplicationsWestern Blotting,Immunohistology - Paraffin,Immunoprecipitation,Flow Cytometry,Immunohistology - Frozen,Immunofluorescence
ConcentrationIgG concentration 0.5 mg/ml
FormatPurified IgG - liquid
ReactivityRat
TargetCD163
IsotypeIgG1
CloneED2
HostMouse
ClonalityMonoclonal Antibody

Questions and Answers

Can this antibody be used to study macrophage activation in rat models?

Yes, the Mouse Anti Rat CD163 antibody has been utilized in various studies to investigate macrophage activation. For instance, it was employed to assess the presence of alternatively activated macrophages in the context of neuroinflammation and spinal cord injury, highlighting its role in identifying specific macrophage phenotypes in rat models ().

What applications has this antibody been shown to work in?

The Mouse Anti Rat CD163 antibody has been shown to work in several applications, including Western Blotting, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Its versatility allows researchers to utilize it in various experimental setups to study macrophage biology and related processes.

Is there any evidence of this antibody being used in studies related to neuroinflammation?

Yes, the antibody has been cited in studies focusing on neuroinflammation, such as its use in characterizing macrophage populations in the injured spinal cord of rats. This research demonstrated the differential expression of pro- and anti-inflammatory markers in macrophages, providing insights into their roles during recovery from spinal cord injury ().

How does this antibody contribute to understanding macrophage behavior in disease models?

The Mouse Anti Rat CD163 antibody is crucial for identifying macrophage populations in various disease models, including those related to neuroinflammation and liver injury. For example, it has been used to analyze macrophage responses in the context of ischemic stroke, where it helped to elucidate the dynamics of macrophage activation and their role in tissue repair and inflammation resolution ().

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Instant Cascara Beverage as a Neuroimmune Modulator of the Brain–Gut Axis: Sex-Dependent Effects in Healthy RatsInternational Journal of Molecular SciencesNovember 3, 2025Paula Gallego-Barceló, Yolanda López-Tofiño, Laura López-Gómez, Gema Vera, Ana Bagues, Jesús Esteban-Hernández, María Dolores del del Castillo, José Antonio Uranga, Raquel AbaloThereafter, sections were incubated overnight at 4 °C with mouse anti-CD163 antibody (clone ED2, BioRad; MCA342GA, 1/1000) to reveal M2 macrophages and rabbit anti-myeloperoxidase (MPO, ab65871, Abcam, 1/1000) to detect

(2) Biofabrication of 3D-printed, pre-cross-linked alginate dialdehyde–gelatin (ADA–GEL) scaffolds for an in vivo metastatic arteriovenous loop tumor modelFrontiers in Bioengineering and BiotechnologyNovember 3, 2025Evelin Sandor, Tannaz Karimi, Rafael Schmid, Yvonne Kulicke, Stefanie Heltmann-Meyer, Carolin Eckert, Sonja K. Schmidt, Jonas Röder, Markus V. Heppt, Aldo R. Boccaccini, et al.Anti-inflammatory macrophages were stained using a mouse monoclonal antibody against CD163 (1:300, clone ED2, MCA342GA, BioRad, diluted with AB Diluent, final concentration of 1.67 µg mL−1) and the Fast Red TR/Naphthol

(3) Effects of Repeated Cisplatin and Monosodium Glutamate on Visceral Sensitivity in RatsCellsDecember 30, 2024Yolanda López-Tofiño, Laura López-Gómez, Marta Martín-Ruíz, Jose Antonio Uranga, Kulmira Nurgali, Gema Vera, Raquel Abaloincubated overnight with the following antibodies: mouse anti-rat CD163 (BioRAD; Alcobendas, Spain; MCA342GA, 1/1000), to quantify M2 macrophages and rabbit anti-rat MPO (Abcam; Amsterdam, The Netherlands; ab65871,

(4) Resistance training suppresses accumulation of senescent fibro-adipogenic progenitors and senescence-associated secretory phenotype in aging rat skeletal muscleGeroScienceSeptember 19, 2024Yung-Li Hung, Ayami Sato, Yuka Takino, Akihito Ishigami, Shuichi Machidaprimary antibodies anti-p21 (ab109199; 1:100)(Abcam), anti-CD68 (ab31630; 1:50)(Abcam), and anti-CD163 (MCA342R; 1:100) (Bio-Rad Laboratories, Hercules, CA, USA) were diluted in 5% NGS/PBS. After incubation with

(5) A vascularized in vivo melanoma model suitable for metastasis research of different tumor stages using fundamentally different bioinksMaterials Today BioJune 1, 2024Rafael Schmid, Sonja K. Schmidt, Stefan Schrüfer, Dirk W. Schubert, Stefanie Heltmann-Meyer, Martin Schicht, Friedrich Paulsen, Raymund E. Horch, Anja K. Bosserhoff, Annika Kengelbach-Weigand, et al.DAPI. CD163 staining was performed as previously published [17]. In short, anti-CD163 (1:200, clone ED2, MCA342GA, Bio- Rad) was used after pretreatment with pronase (Sigma-Aldrich). The detection system was a polymer

(6) Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applicationsMaterials Today BioJune 1, 2024Kest Verstappen, Alexey Klymov, Mónica Cicuéndez, Daniela M. da Silva, Nathalie Barroca, Francisco-Javier Fernández-San-Argimiro, Iratxe Madarieta, Laura Casarrubios, María José Feito, Rosalía Diez-Orejas, et al.trypsin incubation (0.075%) at 37 °C. Samples were incubated with mouse anti-rat CD163 antibody (1:25; MCA342, Bio-Rad, Veenendaal, The Netherlands), followed by incubation with a secondary biotin-conjugated donkey

(7) Sex Dependent Disparities in the Central Innate Immune Response after Moderate Spinal Cord Contusion in RatCellsApril 6, 2024Ghosh M, Lee J, Burke AN, Strong TA, Sagen J, Pearse DD.USA)",ab22048,1:100 Anti-Iba1,Goat,Abcam,ab5076,1:500 "Anti Rat CD68, clone ED1",Mouse,Bio-rad,MCA341R,1:100 Anti-rat CD163,Rabbit,Bio-rad,MCA342R,1:100 "Phospho-IKKα (Ser176, Ser180)",Rabbit,"ThermoFisher Scientific (Waltham, MA, USA)",44-714,1:200 Phospho-IKKβ

(8) A rat liver cell atlas reveals intrahepatic myeloid heterogeneityiScienceNovember 17, 2023Pouyabahar D, Chung SW, Pezzutti OI, Perciani CT, Wang X, Ma XZ, Jiang C, Camat D, Chung T, Sekhon M, et al.Clone: Polyclonal,AbCam,Cat# ab125212; RRID: AB_10975465 Mouse-anti rat CD163; Clone: ED2,BioRad,Cat# MCA342GA ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(9) Alterations in macrophage polarization in the craniofacial and extracranial skeleton after zoledronate application and surgical interventions – an in vivo experimentFrontiers in ImmunologyMay 24, 2023Ann-Kristin Struckmeier, Falk Wehrhan, Raimund Preidl, Melanie Mike, Tina Mönch, Lea Eilers, Jutta Ries, Leah Trumet, Rainer Lutz, Carol Geppert, et al.100-fold diluted anti-CD68 antibody/100-fold diluted anti-CD163 antibody (anti-CD68: MCA341R; anti-CD163: MCA342R, both Bio-Rad Laboratories, Hercules, California, United States) in antibody diluent (DAKO, S2022).

(10) Reversal of Pulmonary Fibrosis: Human Umbilical Mesenchymal Stem Cells from Wharton's Jelly versus Human-Adipose-Derived Mesenchymal Stem CellsInternational journal of molecular sciencesApril 8, 2023Chu KA, Yeh CC, Hsu CH, Hsu CW, Kuo FH, Tsai PJ, Fu YS.USA, 13395-1-AP); and mouse anti-CD163 antibody for labeling M2 macrophage (BioRad, Hercules, CA, USA, MCA342R). Sections were then reacted with secondary antibodies, followed by avidin-biotinylated-HRP complex

(11) Maternal nano-titanium dioxide inhalation alters fetoplacental outcomes in a sexually dimorphic mannerFrontiers in ToxicologyMarch 6, 2023Julie A. Griffith, Allison Dunn, Evan DeVallance, Kallie J. Schafner, Kevin J. Engles, Thomas P. Batchelor, William T. Goldsmith, Kimberley Wix, Salik Hussain, Elizabeth C. Bowdridge, et al.at 4°C. Slides were washed 5 × 5 min, and then incubated in mouse monoclonal anti-rat CD163 (1:500, MCA342R; Bio-Rad Laboratories, Hercules, CA, United States of America) (Rosario et al., 2009) for 24 h at four°C.

(12) Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injuryScientific reportsFebruary 3, 2023Huynh T, Reed C, Blackwell Z, Phelps P, Herrera LCP, Almodovar J, Zaharoff DA, Wolchok J.embryonic myosin heavy chain (eMHC) (F1.652, Hybridoma Bank), CD68 (MA6-13324, Invitrogen), CD163 (MCA342R, Bio-rad), CD3e (MA1-90,582, Invitrogen), collagen I (Col I) (NB600-450, Novus Biologicals), CD31(Pa5-32321,

(13) Pooled human bone marrow-derived mesenchymal stromal cells with defined trophic factors cargo promote dermal wound healing in diabetic rats by improved vascularization and dynamic recruitment of M2-like macrophagesFrontiers in ImmunologyAugust 19, 2022Hélène Willer et al.Abcam, Cambridge, UK, rabbit anti-rat polyclonal CD68 (Ab125212) Abcam, mouse anti-rat monoclonal CD163 (MCA342GA) BioRad, Feldkirchen, Germany, and 1:250 rabbit anti-human monoclonal Ku80 (EPR3468), Abcam). After

(14) Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor functionIBRO Neurosci RepJune 1, 2022Asano K, Nakamura T, Funakoshi K.phase. 2.5.3.1.1 M1 and M2 microglia/macrophages Mouse monoclonal antibodies against CD163 (1:100; MCA342R, Bio-Rad, Hercules, CA, USA) and rabbit polyclonal antibodies against inducible nitric oxide synthase

(15) Effects of Commercial Probiotics on Colonic Sensitivity after Acute Mucosal IrritationInternational Journal of Environmental Research and Public HealthMay 26, 2022Laura López-Gómez, Jaime Antón, Yolanda López-Tofiño, Bianca Pomana, José A. Uranga, Raquel Abaloprimary antibodies: anti-macrophage-associated antigen CD163 (mouse anti-rat CD163, clone ED2, BioRAD; MCA342GA, 1/1000) to quantify the number of proinflammatory macrophages and anti-myeloperoxidase (MPO) to quantify

(16) Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitroScientific reportsMarch 25, 2022Kieu TQ, Tazawa K, Kawashima N, Noda S, Fujii M, Nara K, Hashimoto K, Han P, Okiji T.AngioBio, San Diego, CA, USA), anti-CD68 (MCA341GA, mouse monoclonal; Bio-Rad, Kidlington, UK), anti-CD163 (MCA342GA, mouse monoclonal; Bio-Rad), anti-MHC-II (MCA46R, mouse monoclonal; Bio-Rad), and anti-CD146 (MAB3250,

(17) Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasonePloS oneJanuary 1, 2022Li R, Bhandari S, Martinez-Zubiaurre I, Bruun JA, Urbarova I, Smedsrød B, Simón-Santamaría J, Sørensen KK.positive) by immune labeling with mouse monoclonal anti-rat CD163 (Bio-Rad, Kidlington, UK, Cat. No MCA342GA), and rabbit polyclonal anti-rat glial fibrillary acidic protein (GFAP) (Dako, Glostrup, Denmark, Cat.

(18) Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacereinHistochemistry and cell biologyDecember 1, 2021de Oliveira SA, Cerri PS, Sasso-Cerri E.primary antibodies: mouse anti-CD163 monoclonal IgG antibody (RRID: AB_321966, 1:800, Bio-Rad, USA; code: MCA342R, lot:00,710); rabbit anti-TNF-α polyclonal IgG antibody (RRID: AB_10891701, 1:500, Boster Biological

(19) A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in RatsCellsAugust 17, 2021Garcia GE, Lu YJ, Truong LD, Roncal-Jiménez CA, Miyazaki M, Miyazaki-Anzai S, Cara-Fuentes G, Andres-Hernando A, Lanaspa M, Johnson RJ, et al.macrophages (catalog MCA343GA, AbD Serotec), and CD163+, clone ED2, a marker for M2 macrophages (catalog MCA342R, AbD Serotec) infiltrates. Slides were reacted with mouse anti-rat ED1, anti-rat CD169, and anti-rat

(20) Regenerative Effects of Hypoxia Primed Flowable Placental Formulation in Muscle and Dermal InjuryInternational journal of molecular sciencesJuly 1, 2021Dhall S, Park MS, Li C, Sathyamoorthy M.CA, USA); for CD163, mouse monoclonal (ED-2) anti-CD163 primary antibody for 30 min (6.67 µg/mL) (#MCA342, Biorad, Hercules, CA, USA); for collagen IV, rabbit polyclonal anti-collagen IV primary antibody for

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MOUSE ANTI RAT CD163

MOUSE ANTI RAT CD163

MOUSE ANTI RAT CD163

MOUSE ANTI RAT CD163

MOUSE ANTI RAT CD163

MOUSE ANTI RAT CD163 from Bio-Rad (Formerly AbD Serotec)

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MOUSE ANTI RAT CD163 from Bio-Rad (Formerly AbD Serotec)

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