Immunofluorescence, Extracellular: (Left) COS-7 cells transiently transfected human TNF were stained with TNF Monoclonal Antibody (CSB0050) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TNF Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TNF and stained with control MAb.

Immunofluorescence, Intracellular: (Left) COS-7 cells transiently transfected human TNF were permeabilized and stained with TNF Monoclonal Antibody (CSB0050) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TNF Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TNF and stained with control MAb.

Membrane Proteome Specificity: The specificity of TNF Monoclonal Antibody (CSB0050) was tested on the Membrane Proteome Arrayâ„¢ and shown to be specific for human TNF. The Membrane Proteome Arrayâ„¢ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Arrayâ„¢ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.