Immunofluorescence, Extracellular: (Left) COS-7 cells transiently transfected human CD47 were stained with CD47 Monoclonal Antibody (CSB0037) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD47 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human CD47 and stained with control MAb.

Immunofluorescence, Intracellular: (Left) COS-7 cells transiently transfected human CD47 were permeabilized and stained with CD47 Monoclonal Antibody (CSB0037) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD47 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human CD47 and stained with control MAb.

Membrane Proteome Specificity: The specificity of CD47 Monoclonal Antibody (CSB0037) was tested on the Membrane Proteome Arrayâ„¢ and shown to be specific for human CD47. The Membrane Proteome Arrayâ„¢ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Arrayâ„¢ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.