Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 from Abcam

Product Specs
ItemAnti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab281584
Quantity1 mL, 20 µL, 100 µL
ApplicationsDot, Flow Cyt (Intra), ICC/IF, IP, PepArr, WB
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat
TargetH3C1 citrulline R2 + R8 + R17
ModificationsCitrulline-R17, Citrulline-R17, Citrulline-R17
CloneRM1001
HostRabbit
Antibody TypePrimary Antibody
Gene NameH3C1
ClonalityMulticlonal
Research AreaEpigenetics
IsotypeIgG
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Development of motif-specific monoclonal antibodies for global protein citrullination detection with minimal cross-reactivity to homocitrullinationCell Reports MethodsApril 20, 2026Sophia Laposchan, Erik Riedel, Andrew Flatley, Selina Pasquero, Regina Feederle, Chien-Yun LeeWB Abcam,ab212082,histone H3 (cit R26),rabbit (IgG),monoclonal [EPR20606],not disclosed,"Dot, ELISA, and WB" Abcam,ab281584,histone H3 (cit R2+ cit R8 + cit R17),rabbit (IgG),multiclonal [RM1001],not disclosed,"Dot, Flow Cyt

(2) Galectin-9high Neutrophils Exacerbate Radiation-Induced FrailtyAging CellApril 7, 2026Zhuo Cheng, Le Ma, Yan Chen, Jialun Li, Yali Dai, Wentao Xie, Yingjie Li, Tianlang Xu, Zhihe Hu, Weilin Huang, et al.were Gal‐9 (1:500, Abcam #ab69630), Myeloperoxidase (1:50, Abcam #ab90810), Histone H3 (1:1000, Abcam #ab281584), Ly6g + Ly6c (1:500, Abcam #ab25377), and CD47 (1:500, Abmart #T55251S). 2.3 Flow Cytometry Analysis Mouse

(3) Citrullinated histone H3 identifies neutrophil extracellular trap formation and correlates with renal disease activity in ANCA-associated vasculitisClin Kidney JApril 6, 2026Natsumi Kamijo, Akiko Mii, Takashi Tani, Rei Nakazato, Arimi Ishikawa, Tetsuya Kashiwagi, Ryuji Ohashi, Masataka Kuwana, Akira Shimizu, Yukinao Sakai, et al.formalin-fixed, paraffin-embedded (FFPE) renal biopsy specimens. For H3Cit, two rabbit antibodies, ab281584 (Abcam, Cambridge, UK) and #97272 (Cell Signaling Technology, Danvers, MA, USA), were initially evaluated

(4) Orally Administered Rhamnan Sulfate from Monostroma nitidum Significantly Inhibits Melanoma Metastasis in Lungs and Aorta of Mice Implanted with B16 CellsMarine DrugsMarch 29, 2026Keiichi Hiramoto, Masashi Imai, Masahiro Terasawa, Koji Suzuki1:1000; 551459, BD Biosciences, Franklin Lakes, NJ, USA), citH3 (neutrophil activation marker, 1:1000; ab281584, Abcam), PAD4 (neutrophil activation marker, 1:1000; ab214810, Abcam), and β-actin (1:5000; #58169,

(5) Catalpol mitigates rheumatoid arthritis by targeting neutrophil extracellular trap releaseFront ImmunolMarch 16, 2026Chaoding Li, Liping Wang, Qianyu Li, Wenjie Zhao, Zhengguang Hui, Zhen Zhang, Lu Zhao(Cat# 118165-1-AP), and NE (Cat# AF0010) were obtained from Proteintech. Anti-Cit-H3 antibody (Cat# AB281584) was from Abcam, and anti-MPO antibody (Cat# EM1901-19) was procured from HuaAn Biotechnology. For detection,

(6) NETosis mediates tick blood pool formationJournal of Veterinary Medical ScienceMarch 13, 2026Yuki KOIKE, Hayato KAWADA, Sana SASAKI, Kei JIMBO, Keiko MIZUTANI, Kofi Dadzie KWOFIE, Fusako MIKAMI, Hirotomo KATO, Makoto MATSUBAYASHI, Naotoshi TSUJI, et al.9.0,1:800 CD41,Rabbit monoclonal,Proteintech,24552-1-AP,pH 9.0,"1:5,000" CitH3,Rabbit polyclonal,Abcam,ab281584,pH 6.0,"1:8,000" PSGL-1,Rabbit monoclonal [EPR28170-27],Abcam,ab316113,pH 9.0,"1:10,000" CD62P,Rabbit

(7) Activation and Cell Death of Mouse Eosinophils in Response to Different Microenvironmental StimuliCellsMarch 10, 2026Immaculeta Osuji, Nives ZimmermannMayo clinic; rabbit recombinant multiclonal antibody to citrullinated histone H3 (citH3, Abcam cat#AB281584, Cambridge, UK)) at 4 °C, followed by respective secondary antibodies (goat anti-rat: Alexa Fluor 594,

(8) Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infectionNature ImmunologyMarch 4, 2026Weixiang Liu, Jieming Ping, Lishan Deng, Ke Wang, Jinnuo Xu, Ningxin Peng, Mengyun Yang, Hesen Tang, Zhengjie Liu, Wenliang Wang, et al.(LLSC20230277). Antibodies and reagents Anti-citrullinated histone H3 (citrulline R2 + R8 + R17) (ab5103, 1:1,000 dilution; ab281584, 1:1,000 dilution) and anti-neutrophil elastase (ab314916, 1:100 dilution) were purchased from Abcam.

(9) Computed Tomography Radiomic Signatures Associated With Neutrophil Extracellular Trap Enrichment and First‐Pass Outcome in Ischemic Stroke ThrombiJournal of the American Heart AssociationFebruary 17, 2026Briana A. Santo, Tatsat R. Patel, Seyyed M. Mousavi Janbeh Sarayi, Kerry E. Poppenberg, Sarah Balghonaim, Alexandria Scotti, TaJania D. Jenkins, Vinay Jaikumar, Elad I. Levy, Adnan H. Siddiqui, et al.Indirect immunofluorescence labeling of CitHis (citrullinated histone) was completed using Rabbit Anti Citrullinated Histone (ab281584, 1:200 μg/mL, Abcam, Cambridge, UK), donkey blocking serum, and Donkey Anti Rabbit AF647 secondary antibody

(10) Diabetes Mellitus Facilitates Gallstone Formation Through CXCR2‐NETs–Mediated Liver‐Bile Barrier DamageAdvanced ScienceFebruary 16, 2026Chao Shi, Shuo Feng, Tianming Liu, Ziang Meng, Yi Zheng, Zhenghao Huang, Tong Wang, Bojian Zhang, Dongbo Xue, Biao Ma, et al.(1:200, ab300650, Abcam), Ly6G (1:200, ab210204, Abcam), CXCR2 (1:150, A3301, Abclonal), CitH3 (1:200, ab281584, Abcam), ZO‐1 (1:500, 21773‐1‐AP, Proteintech), occluding (1:500, 27260‐1‐AP, Proteintech) and claudin‐1

(11) pH/ROS‐Responsive Injectable Hydrogel Co‐Loaded with B7‐H3 Blocker and NETs Suppressor Boosts OSCC Synergistic ImmunotherapyAdvanced ScienceFebruary 13, 2026Huan Li, Yun Li, Yating Hu, Yanbing Yao, Yaodong He, Jing Li, Jiaqi Tang, Zhenyan Zhao, Yan Wang, Yulun He, et al.with primary antibodies overnight at 4°C, including anti‐B7‐H3 (#ab227679, 1:100, Abcam), anti‐CitH3 (#ab281584, 1:2000, Abcam), anti‐MPO (#GB11224, 1:1000, Servicebio), anti‐CD4 (#67786‐1‐Ig, 1:200, Proteintech),

(12) Mechanical Strain‐Programmed SDC1 + Sheath Fibroblasts Trigger CXCR4 hi Neutrophil‐Mediated Enthesitis in Ankylosing SpondylitisAdvanced ScienceFebruary 4, 2026Jiajie Lin, Zepeng Su, Yipeng Zeng, Yi Zhou, Chenying Zeng, Weihao Zhang, Qibo Li, Zipeng Xiao, Zibin Chen, Ziqian Liu, et al.anti‐mouse Ly6g (BD, #551459), anti‐mouse/human Cxcr4 (Abcam, #ab124824), anti‐mouse/human H3cit (Abcam, # ab281584), anti‐mouse/human MPO (R&D, #AF3667), donkey anti‐goat IgG Alexa Fluor 647 (Abcam, #ab150131), HRP‐conjugated

(13) Thermosensitive Hydrogel for Controlled Delivery of PAD4 Inhibitor YJ-2 in Diabetic Wound HealingPharmaceuticsJanuary 22, 2026Kai Wang, Ayijiang Taledaohan, Liujia Chan, Yu Lu, Yijiang Jia, Yuji WangTechnology Co., Ltd. (Shanghai, China) Rabbit anti-PAD4 antibody (AB96758), rabbit anti-H3cit antibody (AB281584), rat anti-LY6G antibody (AB210204) were purchased from Abcam (Waltham, MA, USA). Rabbit anti-ACTIN

(14) Ionizing Radiation Induces Extracellular Trap Release from MacrophagesInternational Journal of Molecular SciencesJanuary 19, 2026Yongchan Lee, Monowar Aziz, Ping Wanganti-LAMP1 antibody (cat. No. ab237307), and anti-histone H3 (citrulline R2 + R8 + R17) antibody (cat. no. ab281584) were purchased from Abcam (Waltham, MA, USA). Z-VAD-fmk (final concentration, 20 μM) was purchased

(15) Inflammation-triggered self-immolative conjugates enable oral peptide delivery by overcoming gastrointestinal barriersScience AdvancesJanuary 16, 2026Juan Cheng, Peng Wu, Chenwen Li, Ying Han, Menglong Sun, Yin Dou, Sheng Chen, Jianxiang Zhangantibody (562737, BD Biosciences, USA), anti-CD206 antibody (ab64693, Abcam, USA), anti-CitH3 antibody (ab281584, Abcam, USA), anti-NE antibody (ab314916, Abcam, USA), or anti-FOXP3 antibody (ab215206, Abcam, USA).

(16) Lactate attenuates hepatic ischemia-reperfusion injury by reducing intrahepatic Inflammation and suppressing hepatocyte-neutrophil interaction via the IL-33/ST2 pathwayCell Communication and SignalingJanuary 15, 2026Peiyang Fang, Zihan Zhang, Jingjing Zhu, Haozhe Xu, Xiaotong Han, Nan Xu, Xiyu Wang, Longyang Zhou, Xiaonan Du, Hua Jin, et al.evaluate cell apoptosis. The Reactive Oxygen Species Assay Kit (S0033M, Beyotime, Shanghai, China), CitH3 (ab281584, Abcam) and MPO (ab208670, Abcam) were applied to evaluate neutrophil activation. IL-33 (ab187060, Abcam),

(17) Non-cell-autonomous tumor promotion in DICER1 cancer predispositionDevelopmental CellJanuary 14, 2026Randolph K. Larsen, Jason A. Hanna, Hongjian Jin, Kristin B. Reed, Darden W. Kimbrough, Kyna Vuong, Jongchan Hwang, Grace E. Adkins, Jack D. Hopkins, Bradley T. Stevens, et al.R&D Systems) 1:200 and rabbit-anti-citrullinated histone 3 (#ab5103, Abcam for Figure 6C, 6E, 6H; #ab281584, Abcam for Figure 6J and Figure S5J) 1:200 overnight at 4 °C. The next morning, slides were washed in

(18) Trem1 regulates neutrophil metabolism and recruitment in lung ischemia-reperfusion injuryRedox BiologyJanuary 14, 2026Fengjing Yang, Song Tong, Junhao Wan, Yixing Li, Jiani Gao, Yan Sun, Xiangfu Sun, Huikang Fu, Wenzhuo Luo, Jiayang Xu, et al.(Servicebio, GB12027-100), MPO (Abcam, ab208670), citrullinated histone H3 (CitH3; [RM1001], Abcam, ab281584), CD66B (Sino Biological, S-766-55), JUN (c-Jun Recombinant Superclonal™, 2HCLC; Thermo Fisher), and

(19) A stage specific NETs-related signature in alcoholic steatohepatitis: from molecular subtyping to therapeutic vulnerabilitiesFrontiers in ImmunologyJanuary 14, 2026Wei Gao, Zhiyong Lin, Yuntao Bao, Mingjiang Liu, Guangtao Ma, Xianxiang Chen, Shuiping Yu, Yonglian Zengwere sequentially incubated with anti-MPO (1:5000, Abcam, ab188211) and anti-Histone H3 (1:100, Abcam, ab281584) antibodies, using a tyramide signal amplification (TSA) kit (ABclonal, RK05902) for signal development

(20) Sustained STING-IRF7 signaling aggravates LPS-induced endometrial inflammation via excessive neutrophil extracellular traps generationFrontiers in ImmunologyJanuary 9, 2026Min Chu, Ding Ma, Zhan Song, Li Liang, Fengjuan Xing, Hongchu Bao22225-1-AP, Proteintech, China), and rabbit polyclonal anti-histone H3 (citrulline R2+R8+R17) (1:100, AB281584, Abcam, USA). The expression of IL-17C was detected using rabbit polyclonal anti-IL17C (1:50, A10587,

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Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] from Abcam

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Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] from Abcam

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