Western blot analysis of alpha Internexin in whole tissue lysates. Samples were incubated in alpha Internexin polyclonal antibody (Product # PA3-16725 using a dilution of 1:10,000. Antibody in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.