GAPDH Antibody PA1-16777 from Thermo Fisher Scientific

Product Specs
ItemGAPDH Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-16777
NCBI Gene AliasesGAPD, HEL-S-162eP, G3PD, GAPDH-2, HSD-35, KNC-NDS6, Gapd-s, BARS-38, Gapds, GAPD2, Gapd, HEL-S-278, gapdh-2, GAPDS
ClonalityPolyclonal
ReactivityChicken, Human, Mouse, Non-human primate, Rat
Quantity100 µL
Conjugate/TagUnconjugated
FormatTBS with 0.1% BSA and 0.09% sodium azide
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
TargetGAPDH
HostRabbit
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Concentration0.2 mg/mL
ImmunogenResidues 150 and 200 of human GAPDH
Storage Temperature4° C, do not freeze
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage4°C, do not freeze
IsotypeIgG

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 CleavageToxins (Basel)March 10, 2026Andre F. Batista, Ratnesh Singh, Frank Lee, Shaoqiu Zhuo, Dmitri Leonoudakis, Conor J. Gallaghercat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4

(2) ZMIZ2/MCM3 Axis Participates in Triple-Negative Breast Cancer ProgressionOncology ResearchJanuary 1, 2026Xiaopan Zou, Meiyang Sun, Xin Jiang, Jingze Yu, Xiaomeng Li, Bingyu Nie44-680G; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and GAPDH (1:1000, Cat. No. PA1-16777; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) were added to incubate membranes at 4°C

(3) A murine model of Barth syndrome with cardiac and skeletal muscle selective inactivation of tafazzinDis Model MechMay 6, 2025Erika Yazawa, Erin M. Keating, Suya Wang, Mason E. Sweat, Qing Ma, Yang Xu, Michael Schlame, William T. PumAb, Cell Signaling Technology, 11815S (1:100 dilution); anti-GAPDH rabbit mAb, Life Technologies, PA116777 (1:100 dilution); and OxPhos Rodent WB Antibody Cocktail (Thermo Fisher Scientific, 45-8099). mRNA

(4) ELAVL1-dependent SOAT2 exacerbated the pancreatitis-like cellular injury of AR42J cells induced by hyperstimulation with caeruleinThe Kaohsiung journal of medical sciencesJanuary 1, 2025Sun YJ, Chen HY, Lai XQ.Thermo Fisher), HO‐1 (#PA5‐77833, 1:1000, Thermo Fisher), GPX4 (#MA5‐32827, 1:10000, Thermo Fisher) and GAPDH (#PA1‐16777, 1:5000, Thermo Fisher). The PVDF membranes were placed in ECL working solution (Beyotime) and then

(5) Specific catalytically impaired DDX3X mutants form sexually dimorphic hollow condensatesNature communicationsNovember 5, 2024Owens MC, Shen H, Yanas A, Mendoza-Figueroa MS, Lavorando E, Wei X, Shweta H, Tang HY, Goldman YE, Liu KF.MA1-91878-HRP, 1:1,000), rabbit anti-DDX3Y (Invitrogen PA5-90055, 1:3,000), rabbit anti-GAPDH (Invitrogen PA1-16777, 1:1,000)]. After washing three times with 1× PBST, the horseradish peroxidase (HRP)-conjugated secondary

(6) The n-Butanol Extract Obtained from the Inner Bark of Tabebuia rosea (Bertol.) DC, Specioside, and Catalposide Induce Leukemia Cell Apoptosis in the Presence of ApicidinMoleculesAugust 23, 2024Nancy Yadira Guerrero-Pepinosa, Luz Angela Veloza, Juan Carlos Sepúlveda-Ariasbcl-2 (1:2000 dilution, PA1-28275), Bax (1:2000 dilution, PA5-70418), and GAPDH (1:10,000 dilution, PA1-16777) antibodies overnight at 4 °C. All the antibodies were purchased from Invitrogen-Thermo Fisher Scientific

(7) CNPY2 governs PDGF‑BB‑treated vascular smooth muscle cell proliferation, migration and phenotypic transformation via the Akt/mTOR/GSK‑3β signaling pathwayExperimental and therapeutic medicineMay 1, 2024Sun H, Wang X, Ma L, Li X, Jin W, Yang Y.Proteintech), p-GSK-3β (67558-1-Ig; 1:5,000; Proteintech), GSK-3β (22104-1-AP; 1:4,000; Proteintech) and GAPDH (PA1-16777; 1:5,000; Invitrogen; Thermo Fisher Scientific, Inc.). The membranes were washed with TBST (0.05% Tween

(8) Elevated α-synuclein levels inhibit mitophagic fluxNPJ Parkinson's diseaseApril 9, 2024Kinnart I, Manders L, Heyninck T, Imberechts D, Praschberger R, Schoovaerts N, Verfaillie C, Verstreken P, Vandenberghe W.

(9) Genetic modifiers modulate phenotypic expression of tafazzin deficiency in a mouse model of Barth syndromeHuman molecular geneticsJune 5, 2023Wang S, Yazawa E, Keating EM, Mazumdar N, Hauschild A, Ma Q, Wu H, Xu Y, Shi X, Strathdee D, et al.

(10) Hsa_circ_0084003 modulates glycolysis and epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma through targeting hsa-miR-143-3p/DNMT3A axisToxicology researchJune 1, 2023Wang K, Chen Z, Qiao X, Zheng J.

(11) Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer CellsInternational journal of molecular sciencesJanuary 9, 2023Jacksi M, Schad E, Buday L, Tantos A.Germany), EHF (PA5-84038, Life Technologies, Paisley, UK), ACTB (MA1-140, Life Technologies), and GAPDH (PA1-16777, Life Technologies). Goat anti-rabbit IgG (H+L) superclonal secondary antibody (A27036, Life Technologies)

(12) The impact of a maternal and offspring obesogenic diet on daughter's oocyte mitochondrial ultrastructure and bioenergetic responses. Insights from an outbred mouse modelFrontiers in physiologyJanuary 1, 2023Xhonneux I, Marei WFA, Meulders B, Andries S, Leroy JLMR.total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413) and GAPDH Antibody (Thermo Fisher Scientific™, US, PA1-16777) as a housekeeping protein for relative quantification. After lysis of denuded oocytes in lysis buffer

(13) DYRK1A inhibitors leucettines and TGF-β inhibitor additively stimulate insulin production in beta cells, organoids, and isolated mouse isletsPloS oneJanuary 1, 2023Pucelik B, Barzowska A, Czarna A.

(14) MiR-30b-5p attenuates the inflammatory response and facilitates the functional recovery of spinal cord injury by targeting the NEFL/mTOR pathwayBrain and behaviorDecember 1, 2022Zhang H, Wang D, Tong J, Fang J, Lin Z.p70S6K (PA5‐28597, 1:1000, Thermo Fisher) and p‐p70S6K (PA5‐38307, 1:1000, Thermo Fisher) with GAPDH (PA1‐16777, 1:2000, Thermo Fisher) as the internal control. After washing 3 times with Tris‐buffered saline‐Tween

(15) Detection of exogenous DNA uptake by murine dendritic cellsSTAR protocolsSeptember 16, 2022Celias DP, de Mingo Pulido Á, Ruffell B.

(16) TRPM2 regulates autophagy to participate in hepatitis B virus replicationJournal of viral hepatitisAugust 1, 2022Chen L, Zhu L, Lu X, Ming X, Yang B.

(17) Sexually dimorphic RNA helicases DDX3X and DDX3Y differentially regulate RNA metabolism through phase separationMolecular cellJuly 21, 2022Shen H, Yanas A, Owens MC, Zhang C, Fritsch C, Fare CM, Copley KE, Shorter J, Goldman YE, Liu KF.anti-FLAG,Invitrogen,"Cat# MA1-91878-HRP, RRID:AB_2537626" Rabbit polyclonal anti-GAPDH,Invitrogen,"Cat# PA1-16777, RRID:AB_568552" Mouse monoclonal anti-GFP,Santa Cruz,"Cat# sc-996, RRID:AB_2187785" "Mouse anti-puromycin,

(18) Contact-dependent signaling triggers tumor-like proliferation of CCM3 knockout endothelial cells in co-culture with wild-type cellsCellular and molecular life sciences : CMLSJune 4, 2022Rath M, Schwefel K, Malinverno M, Skowronek D, Leopoldi A, Pilz RA, Biedenweg D, Bekeschus S, Penninger JM, Dejana E, et al.ab109520, Abcam), Anti-p53 (1:40, sc-126, Santa Cruz Biotechnology, Dallas, TX, USA) and Anti-GAPDH (1:500, PA1-16777, Thermo Fisher Scientific). A HRP-conjugated goat anti-rabbit immunoglobulin antibody (1:400, ab205718,

(19) Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5pThoracic cancerJune 1, 2022Li D, Fu Z, Dong C, Song Y.Invitrogen), vimentin (#10366‐1‐AP, 1:3000, Invitrogen), PFN2 (#PA5‐21959, 1:1000, Invitrogen), GAPDH (#PA1‐16777, 1:4000, Invitrogen), NOVA2 (#PA5‐69076, 1.25 μg/ml, Invitrogen), Lamin A/C (#MA3‐1000, 1:500, Invitrogen),

(20) Identification of a STIM1 Splicing Variant that Promotes Glioblastoma GrowthAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2022Xie J, Ma G, Zhou L, He L, Zhang Z, Tan P, Huang Z, Fang S, Wang T, Lee YT, et al.(#sc‐8334) and β‐actin (#sc‐47778), purchased from Santa Cruz Biotechnology (Dallas, TX). Anti‐GAPDH (#PA1‐16777) was purchased from Thermo Fisher Scientific (Waltham, MA). Mouse monoclonal STIM1 antibody and anti‐STIM1

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GAPDH Antibody

GAPDH Antibody from Thermo Fisher Scientific

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GAPDH Antibody from Thermo Fisher Scientific

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