Western blot analysis of ASC1 in different various lysates. Lane 1: A549 cell lysate; Lane 2: Hela cell lysate; Lane 3: U87MG cell lysate; Lane 4: 293T cell lysate; Lane 5: HL-60 cell lysate; Lane 6: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 66 kDa. Observed band size: 66 kDa. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Primary antibody ASC1 polyclonal antibody (Product # PA5-144669) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.