Immunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue using M9769-97E, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of M9769-97E for immunohistochemistry.

Fluorescent confocal image of Hela cell stained with M9769-97E. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with M9769-97E (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10 min). MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.