Immunohistochemical analysis of of paraffin-embedded human breast tissue using O8057-07. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA buffer, pH 9. 0. Samples were incubated with O8075-07 (1:100) for 1 hour at RT. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Confocal Immunofluorescence analysis of in A375 cells using O8057-07 followed by Alexa Fuor 488-conjugated goat anti-rabbit IgG (green). DAPI was used to stain the cell nuclear (blue).