Western Blot analysis using 034958 (1:2000) Lane 1: M. stomach tissue lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000) Predicted band size: 134kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry of paraformaldehyde-fixed, paraffin-embedded human heart tissue sections using 034958. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with 034958 (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemistry of paraformaldehyde-fixed, paraffin-embedded human heart kidney sections using 034958. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with 034958(1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemistry analysis in formalin fixed paraffin embedded human brain tissue using 034958 followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of 034958 for immunohistochemistry.