Western Blot analysis: All lanes: 035287 (1:2000) Lane 1: Jurkat whole cell lysates Lane 2: MDA-MB-231 whole cell lysates Lane 3: MDA-MB-468 whole cell lysates Lane 4: T47D whole cell lysates Lane 5: mouse spleen lysates Lysates/proteins (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (1:10,000 dilution) Predicted band size: 85kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemisty analysis in formalin-fixed and paraffin-embedded human testis tissue reacted with 035287, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of 035287 for immunohistochemistry.

Confocal immunofluorescent analysis of 293 cells using 035287 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow Cytometric analysis of Jurkat cells using 035287 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.