Immunohistochemistry analysis in formalin, fixed paraffin embedded human breast carcinoma using 043650 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of 043650 for immunohistochemistry.

Fluorescent confocal image of SK-BR-3 cell stained with 043650. SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with 043650 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.