Western blot analysis of 045846. Lane 1: Non-transfected HEK293 crude homogenate. Lane 2: Crude homogenate of transfected HEK293 cells which overexpress protein EosFP. Lane S: Protein standard of indicated molecular weight 045846 at 1:1,000 dilution reveals a strong clean band at ~25 kDa corresponding to EosFP in transfected cells, which is absent from non-transfected cells.