Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded human cardiac muscle (Fig. 1 & 2) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:50 dilution of Myosin XIX (Myo19). Reactivity was detected using the IHC-Select® Detection Kit. Staining pattern appears as cytoplasmic. Staining was observed in human cardiac muscle.