Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using 032134 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with 032134 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.