V5 Tag Antibody PA1-993 from Thermo Fisher Scientific

Product Specs
ItemV5 Tag Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-993
ClonalityPolyclonal
ApplicationsWestern Blot
ReactivityTag
FormatPBS with 1mg/mL BSA and 0.05% sodium azide
Conjugate/TagUnconjugated
IsotypeIgG
TargetV5 Tag
HostRabbit
Quantity100 µg
ImmunogenSynthetic peptide corresponding to residues G K P I P N P L L G L D S T
Concentration1 mg/mL
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Targeting ceramide synthases for the development of new antifungalsStructureSeptember 4, 2025Deveney Dasilva, Nivea Pereira de Sa, Kathryn Takemura, Kalani Jayanetti, Jeehyun Karen You, Nathalia Vieira de Sa, Gabriel Soares Matos, Andy Zhong, Can E. Senkal, Yusuf Hannun, et al.analysis using primary antibody primary antibody V5 Tag Polyclonal Antibody (Life Technologies: Cat# PA1–993) and secondary antibody Goat anti-Rabbit IgG (heavy chain) HRP (Life Technologies: Cat# A27036) (Supplementary

(2) Engineered ipilimumab variants that bind human and mouse CTLA-4MAbsJanuary 24, 2025Brett Robison, S J Diong, Anusha Kumar, Thomas M. Moon, Olin Chang, Bryant Chau, Christine Bee, Ishita Barman, Arvind Rajpal, Alan J. Korman, et al.libraries were subjected to primary labeling using Rabbit anti-V5 (ThermoFisher Scientific Cat. No. PA1–993) to detect light chain display and biotinylated His-Avi tagged mCTLA-4 extracellular domain followed

(3) FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell CytotoxicityCOPD: Journal of Chronic Obstructive Pulmonary DiseaseDecember 31, 2024Xiuying Li, Sowmya P. Lakshmi, Kiyoshi Uemasu, Zachary Lane, Rajan T. Reddy, Divay Chandra, Chunbin Zou, Yu Jiang, Toru Nyunoyaselenium, 10% fetal bovine serum. Cells were cultured in a 5% CO2 incubator at 37°C. V5 antibody (#PA1–993) was purchased from Invitrogen (St. Louis, MO). STK11 (#3050) antibody was purchased from Cell Signaling

(4) Lipid- and phospho-regulation of CTP:Phosphocholine Cytidylyltransferase α association with nuclear lipid dropletsMolecular biology of the cellMarch 1, 2024Foster J, McPhee M, Yue L, Dellaire G, Pelech S, Ridgway ND.2020), Lipin1 (Abcam, ab181389), OSBP (Zhao and Ridgway, 2017) and V5 (BioRad, MCA1360GA or Invitrogen, PA1-993). After removal of primary antibodies, membranes were washed twice and incubated for 1 h at room temperature

(5) Re-examining the role of the dorsal fan-shaped body in promoting sleep in DrosophilaCurrent biology : CBSeptember 11, 2023De J, Wu M, Lambatan V, Hua Y, Joiner WJ.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rabbit anti-V5,Thermo Fisher,PA1993 Rabbit anti-HA,Rockland,RL600-401-384 Rat anti-HA,Roche,11867423001 Rabbit anti-GFP,"Invitrogen,

(6) Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopeLife science allianceSeptember 1, 2023Cheng LC, Zhang X, Baboo S, Nguyen JA, Martinez-Bartolomé S, Loose E, Diedrich J, Yates JR 3rd, Gerace L.antibodies were used for immunofluorescence staining: mouse anti-V5 (#46-0705; Invitrogen), rabbit anti-V5 (#PA1-993; Thermo Fisher Scientific), rabbit anti-calnexin (#ab22595; Abcam), guinea pig anti-lamin A/C (made

(7) Expression of the excitatory opsin ChRERα can be traced longitudinally in rat and nonhuman primate brains with PET imagingScience translational medicineJuly 26, 2023Bonaventura J, Boehm MA, Jedema HP, Solis O, Pignatelli M, Song X, Lu H, Richie CT, Zhang S, Gomez JL, et al.blocking buffer was then removed and replaced with primary antibody (V5 Rabbit Polyclonal, Invitrogen PA1-993) diluted in LICOR blocking buffer (1:1500) and incubated overnight at 4°C. On the second day, the membrane

(8) Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxivJune 13, 2023Li-Chun Cheng, Xi Zhang, Sabyasachi Baboo, Julie A Nguyen, Salvador Martinez-Bartolomé, Esther Loose, Jolene Diedrich, John R Yates, Larry Geracefor immunofluorescence staining: mouse anti-V5 (Invitrogen #46-0705), rabbit anti-V5 (ThermoFisher #PA1-993), rabbit anti-calnexin (Abcam #ab22595), guinea pig anti-lamin A/C (made in-house to gel-purified rat

(9) The pUL51 Tegument Protein Is Essential for Marek's Disease Virus Growth In Vitro and Bears a Function That Is Critical for Pathogenesis In VivoJournal of virologyMay 31, 2023Pasdeloup D, Chuard A, Rémy S, Courvoisier-Guyader K, Denesvre C.

(10) A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in DrosophilaCell Reports MethodsMay 22, 2023Michael J. Parisi, Michael A. Aimino, Timothy J. Mosca1:100), mouse anti-V5 (ThermoFisher, cat. no. MA515253, 1:100), rabbit anti-V5 (ThermoFisher, cat. no. PA1993, 1:100), rabbit anti-V5 (Cell Signaling, cat. no. 13202S, 1:100), Alexa647-conjugated goat anti-HRP

(11) Cigarette smoking is a secondary cause of folliculin lossThoraxApril 1, 2023Xiuying Li, Yandong Lai, Zachary Lane, Hilary Strollo, Kazuya Tanimura, John C Sembrat, Chunbin Zou, Michael M Myerburg, Mauricio Rojas, Steven Shapiro, et al.(RIPA) Lysis and Extraction Buffer (Cat#: 89901), ataxia‐telangiectasia mutated (ATM) siRNA (Cat#: 1299001), V5 antibody (Cat#: PA1-993) and Escherichia coli Top10 competent cells (Cat#: C404010) were purchased from Invitrogen (St Louis,

(12) The SUMO ligase MMS21 profoundly influences maize development through its impact on genome activity and stabilityPLoS geneticsOctober 1, 2021Zhang J, Augustine RC, Suzuki M, Feng J, Char SN, Yang B, McCarty DR, Vierstra RD.polyclonal antibody coupled to HRP (Invtrogen; Cat. No. PA1-33133), and the V5 tag (Invitrogen; Cat. No. PA1-993). RNA isolation, qRT-PCR, and cDNA cloning Total RNA was isolated from 10-DAS shoots using the RNeasy

(13) Identification of protein interactions of grapevine fanleaf virus RNA-dependent RNA polymerase during infection of Nicotiana benthamiana by affinity purification and tandem mass spectrometryJournal of General VirologyMay 27, 2021Larissa J. Osterbaan, Victoria Hoyle, Michelle Curtis, Stacy DeBlasio, Keith D. Rivera, Michelle Heck, Marc Fuchsapical leaves, as shown by SDS-PAGE and western blot detection with an anti-V5 antibody (Invitrogen PA1-993) (Fig. 1b, red arrowhead). In addition, an anti-V5 immunoreactive protein band of higher molecular mass

(14) Differential activation of JAK-STAT signaling reveals functional compartmentalization in Drosophila blood progenitorseLifeFebruary 17, 2021Diana Rodrigues, Yoan Renaud, K VijayRaghavan, Lucas Waltzer, Maneesha S InamdarMolecular Probes Inc), rabbit anti-DsRed (1:200, Takara Bio.), rabbit anti-V5 (1:1000, ThermoFisher #PA1-993), mouse anti-ProPO antibody (1:5, Bioneeds), and rabbit anti-Asrij (1:50, [Kulkarni et al., 2011]).

(15) Functional α6β4 acetylcholine receptor expression enables pharmacological testing of nicotinic agonists with analgesic propertiesThe Journal of clinical investigationNovember 2, 2020Knowland D, Gu S, Eckert WA 3rd, Dawe GB, Matta JA, Limberis J, Wickenden AD, Bhattacharya A, Bredt DS.Cell Signaling Technology [3294S]; anti-HA, Thermo Fisher Scientific [26183]; anti-V5, Thermo Fisher Scientific [PA1993]; and anti-BARP [ref. 13]). Blots were then incubated for 1 hour at room temperature in the dark with

(16) Targeted integration by homologous recombination enables in situ tagging and replacement of genes in the marine microeukaryote Diplonema papillatumEnvironmental microbiologySeptember 1, 2020Faktorová D, Kaur B, Valach M, Graf L, Benz C, Burger G, Lukeš J.

(17) PKCλ/ι Loss Induces Autophagy, Oxidative Phosphorylation, and NRF2 to Promote Liver Cancer ProgressionCancer cellAugust 10, 2020Kudo Y, Sugimoto M, Arias E, Kasashima H, Cordes T, Linares JF, Duran A, Nakanishi Y, Nakanishi N, L'Hermitte A, et al.anti-Thiophosphate ester,Abcam,"Cat# ab92570, RRID:AB_10562142" Rabbit anti-V5 tag,Thermo Fisher Scientific,"Cat# PA1–993, RRID:AB_561893" Rabbit anti-ZEB1,Santa Cruz Biotechnology,"Cat# sc-25388, RRID:AB_2217979" Rat anti-B220

(18) HIV-1 Vpr induces cell cycle arrest and enhances viral gene expression by depleting CCDC137eLifeJune 15, 2020Zhang F, Bieniasz PD.and anti-V5 (Invitrogen, R960-25). Polyclonal antibodies were purchased from ThermoFisher (anti-V5, PA1-993), or Abcam (anti-CC137, ab185368 or ab183864; anti-gamma H2AX phosphor S139, ab11174; anti-Fibrillarin,

(19) Cigarette smoke exposure enhances transforming acidic coiled-coil-containing protein 2 turnover and thereby promotes emphysemaJCI insightJanuary 30, 2020Mallampalli RK, Li X, Jang JH, Kaminski T, Hoji A, Coon T, Chandra D, Welty S, Teng Y, Sembrat J, et al.obtained from Santa Cruz Biotechnology Inc. Immobilized protein A/G beads (catalog 20421), V5 (catalog PA1-993), the pcDNA3.1D-V5-His-TOPO cloning kit (catalog 45-0158), and TOP10 competent cells (catalog C404010)

(20) Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cellsNucleus (Austin, Tex.)December 1, 2019Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolomé S, Tapia O, Zhang X, Yates JR 3rd, Gerace L.immunofluorescence staining: Primary antibodies: mouse anti-V5 (Invitrogen #46–0705), rabbit anti-V5 (Thermo Fisher #PA1-993), rabbit anti-calnexin (Abcam #ab22595), guinea pig anti-lamin A (made in-house), mouse anti-NPC RL1

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V5 Tag Antibody

V5 Tag Antibody from Thermo Fisher Scientific

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V5 Tag Antibody from Thermo Fisher Scientific

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