All lanes: 038758 (1:2000) Lane 1: Human skeletal muslce lysate Lane 2: HL-60 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: RD whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat at 1:10,000. Predicted band size: 35kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of Hela cell using 038758. Hela cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with 038758 (1:25, 1hr at 37ºC). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37ºC). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10mins). MyoD1 immunoreactivity is localized to nucleus significantly.