Western Blot analysis in HepG2 cell line lysates (35ug/lane) using 035338. This demonstrates that 035338 detected the FADS2 protein (arrow).

Immunohistochemistry analysis in formalin fixed paraffin embedded human liver tissue using 035338 followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of 035338 for immunohistochemistry.

Fluorescent image of A549 cell stained with 035338. A549 cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with 035338 (1:25, 1hr at 37ºC). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37ºC).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1hr at 37ºC).FADS2 immunoreactivity is localized to cytoplasm and vesicles significantly.