Sandwich ELISA: Solid phase was coated with 3 ug/ml anti CEA binding human CEACAM1- CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody mAb C5-1X (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.

Flow cytometry: 10 ug/ml of primary mAb C5-1X; 250.000 Hela-human CEACAM1 cells.