Western Blot analysis using 033986 (1:2000) Lane 1: Hela whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 66kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of formalin fixed paraffin embedded human skeletal muscle using 033986. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA buffer, pH 9. 0. Samples were incubated with 033986 (1:250) for 15 minutes at RT.

Immunohistochemistry analysis of formalin fixed paraffin embedded human liver using 033986. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA buffer, pH 9. 0. Samples were incubated with 033986 (1:250) for 15 minutes at RT.