Western Blot using G7121-04N (1:2000) Lane 1: mouse brain lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Western Blot using G7121-04N (1:2000) Lane 1: HT-29 whole cell lysates Lane 2: mouse cerebellum lysates Lane 3: Raji whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human testis tissue using G7121-04N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunohistochemistry analysis in human liver tissue sections using G7121-02N. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with G7121-04N (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.