Vaccinia Virus Antibody PA1-7258 from Thermo Fisher Scientific

Product Specs
ItemVaccinia Virus Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-7258
ClonalityPolyclonal
FormatPBS with 0.1% sodium azide; pH 7.2
TargetVaccinia Virus
HostRabbit
ApplicationsELISA, Immunohistochemistry
Concentration5 mg/mL
ImmunogenNew York City Board of Health (NYCBOH) strain
IsotypeIgG
Storage TemperatureMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
PurityProtein A
Antibody TypePrimary
ReactivityVirus (Vi)
Conjugate/TagUnconjugated
Quantity1 mL

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Plant‐based production and characterization of monoclonal antibodies targeting extracellular enveloped proteins of the Mpox VirusBiotechnology ReportsSeptember 1, 2026Kitti Jirananon, Balamurugan Shanmugaraj, Suwimon Manopwisedjaroen, Arunee Thitithanyanont, Prinjaporn Teengam, Waranyoo PhoolcharoenBiotech, USA) at 37 °C for 1 h. For positive control, rabbit polyclonal anti-vaccinia antibody (1:1000, PA17258, ThermoFisher Scientific, USA) was applied, followed by goat anti-rabbit HRP (1:5000, ThermoFisher Scientific,

(2) DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responsesJ Exp MedMay 4, 2026Isabelle K. Vila, Yasmine Messaoud-Nacer, Clara Taffoni, Jane Jardine, Roger J. Eloiflin, Adeline Augereau, Soumyabrata Guha, Moritz Schussler, Pierre Le Hars, Joe McKellar, et al.Sciences), washed, and immunostained for MPXV antigens with rabbit polyclonal anti-VACV antibodies (PA1-7258, Invitrogen) and an Alexa Fluor 488-coupled goat anti-rabbit antibody (Invitrogen). Nuclei were stained

(3) Poxvirus infection triggers remodeling of host m⁶A epitranscriptome and benefits from the m⁶A regulatory responsesVirology JournalApril 11, 2026Taehyung Kwon, Demosthenes P. Morales, Abigale S. Mikolitis, Phillip M. Mach, Cheryl D. Gleasner, Sofiya N. Micheva-Viteva(NBP1-19088, Novus Bio, Centennial, CO), anti-YTHDF1 (P5HB-23, Invitrogen-TFS), and anti-Vaccinia virus (PA1-7258, Invitrogen-TFS). Alexa Fluor488 or Alexa Fluor555 goat anti-rabbit IgGs were used to fluorescently

(4) An improved and high-throughput mpox virus microneutralization assayBiosafety and HealthApril 1, 2026Danyang Li, Zuoyuan Du, Qiao Zhang, Rui Song, Lan Chen, He Huang, Jianwei Wang, Li Guo, Lili RenVaccinia virus polyclonal antibody targeting vaccinia virus B5 and A33 protein (Invitrogen, Cat no. PA1-7258) was used as a positive control. 2.6 Indirect IFA Vero cells were infected with MPXV in a BSL-3 laboratory

(5) Universal and quantitative detection of double-stranded RNAs as a signature of pan-virus infections using a luciferase-based biosensorJournal of Biological EngineeringDecember 16, 2025Michihito Sasaki, Eri Fujii, Satoko Sasaki, Takuma Ariizumi, Kei Konishi, Akihiko Sato, William W. Hall, Hirofumi Sawa, Yasuko Orbaanti-RVA goat polyclonal (AB1129, Merck), anti-Vaccinia virus rabbit polyclonal (cross reactive with MPXV; PA17258, Thermo Fisher Scientific), anti-HSV-1 rabbit polyclonal (B0114, DAKO), anti-JEV rabbit polyclonal (serum

(6) Structural insights into tecovirimat antiviral activity and poxvirus resistanceNature MicrobiologyFebruary 12, 2025Riccardo Vernuccio, Alejandro Martínez León, Chetan S. Poojari, Julian Buchrieser, Christopher N. Selverian, Yakin Jaleta, Annalisa Meola, Florence Guivel-Benhassine, Françoise Porrot, Ahmed Haouz, et al.after 48 h with 4% paraformaldehyde and washed and immunostained with polyclonal anti-VACV antibodies (PA1-7258, Invitrogen) and an Alexa Fluor 488-coupled goat anti-rabbit antibody (CA-11008, Invitrogen). Images

(7) Experimental inoculation of pigs with monkeypox virus results in productive infection and transmission to sentinelsEmerging microbes & infectionsDecember 1, 2024Mantlo E, Trujillo JD, Gaudreault NN, Morozov I, Lewis CE, Matias-Ferreyra F, McDowell C, Bold D, Kwon T, Cool K, et al.minutes and washed twice with PBS. Next, 1 mL of rabbit anti-vaccinia virus (VACV) polyclonal antibody (PA1-7258, Invitrogen, Waltham, MA, USA) diluted 1:250 in 1% bovine serum albumin (BSA) in PBS was added per well,

(8) Comparative Analysis of 2022 Outbreak MPXV and Previous Clade II MPXVJournal of Medical VirologyNovember 1, 2024Joseph Patrick McGrail, Alberto Paniz Mondolfi, Juan David Ramírez, Santiago Vidal, Adolfo García‐Sastre, Gustavo Palacios, Mari Paz Sanchez‐Seco, Susana GuerraPBS for 30 min, and blocked in PBS with 10% FCS for 30 min at room temperature. Poli‐VACV (Invitrogen PA1‐7258), a policlonal antibody was used as the primary antibody. Alexa Fluor 488‐conjugated rabbit IgG antibodies

(9) Complementary dual-virus strategy drives synthetic target and cognate T-cell engager expression for endogenous-antigen agnostic immunotherapyNature communicationsAugust 23, 2024Taha Z, Crupi MJF, Alluqmani N, MacKenzie D, Vallati S, Whelan JT, Fareez F, Alwithenani A, Petryk J, Chen A, et al.

(10) Smallpox vaccination campaigns resulted in age-associated population cross-immunity against monkeypox virusJournal of General VirologyJune 11, 2024Kieran Dee, Maria Manali, Laura A. Bissett, Jordan Bone, Callum Magill, Chris Davis, Brian J. Willett, Pablo R. Murciamin. Virus positive cells were detected using a polyclonal antibody raised against VACV (Invitrogen: PA1-7258) at a dilution of 1 : 1000 and then a secondary antibody (goat anti-rabbit IgG Alexa Flour 488, A11034)

(11) Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individualsCell host & microbeJune 14, 2023Hubert M, Guivel-Benhassine F, Bruel T, Porrot F, Planas D, Vanhomwegen J, Wiedemann A, Burrel S, Marot S, Palich R, et al.paraformaldehyde, washed and immunostained for MPXV antigens with rabbit polyclonal anti-VACV antibodies (PA1-7258, Invitrogen), and an Alexa Fluor 488-coupled goat anti-rabbit antibody (Invitrogen). Nuclei were stained

(12) The ectromelia virus virulence factor C15 facilitates early viral spread by inhibiting NK cell contactiScienceDecember 22, 2022Peauroi EM, Carro SD, Pei L, Reynoso GV, Hickman HD, Eisenlohr LC.

(13) Modified vaccinia Ankara expressing EEHV1A glycoprotein B elicits humoral and cell-mediated immune responses in micePLOS ONEMarch 21, 2022Taylor Pursell, Jennifer L. Spencer Clinton, Jie Tan, Rongsheng Peng, Paul D. Lingmethanol:acetone, and blocked with PBS/4% FBS for 1 hour. Rabbit anti-vaccinia antibody (Invitrogen, PA1-7258) was diluted in PBS/4% FBS (1:500) and incubated with cells for 1.5 hours at room temperature (RT).

(14) Single Immunization of a Vaccine Vectored by a Novel Recombinant Vaccinia Virus Affords Effective Protection Against Respiratory Syncytial Virus Infection in Cotton RatsFrontiers in immunologyJanuary 1, 2021Russell MS, Thulasi Raman SN, Gravel C, Zhang W, Pfeifle A, Chen W, Van Domselaar G, Safronetz D, Johnston M, Sauve S, et al.virus was immune-titrated on DF-1 cells with Rabbit Anti-Vaccinia Virus Polyclonal Ab from Thermo (Cat# PA17258) as the primary antibody followed by the secondary antibody, ECL Donkey Anti-Rabbit HRP (Amersham Cat#NA934V)

(15) Ectromelia-encoded virulence factor C15 specifically inhibits antigen presentation to CD4+ T cells post peptide loadingPLoS pathogensAugust 1, 2020Forsyth KS, Roy NH, Peauroi E, DeHaven BC, Wold ED, Hersperger AR, Burkhardt JK, Eisenlohr LC.0.5% Triton-X in PBS, and blocking with 5% BSA in TBS-T. Wells were stained with Rb anti-VACV Ab (Thermo PA1-7258) followed by HRP Gt anti-Rb secondary (CST 7074S). Lastly wells were incubated with KPL TrueBlue TMB

(16) Organism-Level Analysis of Vaccination Reveals Networks of Protection across TissuesCellOctober 5, 2017Kadoki M, Patil A, Thaiss CC, Brooks DJ, Pandey S, Deep D, Alvarez D, von Andrian UH, Wagers AJ, Nakai K, et al.immunostaining for MVA using rabbit polyclonal anti-vaccinia virus primary antibody (ThermoFisher Scientific PA1-7258) and HRP-Goat anti-rabbit secondary antibody (ThermoFisher Scientific 656120) diluted at 1:1000 in PBS

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Vaccinia Virus Antibody from Thermo Fisher Scientific

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Vaccinia Virus Antibody from Thermo Fisher Scientific

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