Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded normal human brain (Fig. 1) and human prostate (Fig. 2) tissues were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:1,000 - 2,000 dilution Anti-Calnexin. Reactivity was detected using the IHC-Select® Detection Kit. Positive staining was observed in both human brain and prostate sections. Staining was very ubiquitous across many of the tissues tested, as it should be with this target.