Western blot analysis of different tissue or cell lysates using 048662 (1:5000) in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kD band present in all preparations corresponds to HSP60 protein. The blot was simultaneously probed with mouse mAb to HSP27, (031373) 1:10,000, in green. Strong single band at ~27kD corresponds to the HSP27 protein, detected only in human cell lines since this particular antibody does not recognize rodent HSP27.