Western blot analysis of Jurkat whole cell lysate (20 µg). Lane A probed with Rat anti tubulin alpha antibody (VMA00051) at a 1/1000 dilution followed by detection with Dylight550 conjugated Rabbit F(ab’)2 anti Rat IgG (STAR16D550) at a 1/10,000 dilution. Lane B probed with Rabbit F(ab’)2 anti Rat IgG (STAR16D550) at a 1/10,000 dilution. Performed under reduced conditions. Visualized on ChemiDoc Touch imaging system. Arrow points to tubulin alpha (molecular weight 52kDa).