Western Blot analysis using 032116 at 1:2000 Lane 1: HepG2 whole cell lysate Lane 2: Human skeletal muscle tissue lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 41kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using 032116 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT, antigen retrieval was by heat mediation with a EDTA buffer, pH 9.0. Samples were incubated with 032116 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using 032116 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT, antigen retrieval was by heat mediation with a EDTA buffer, pH 9. 0. Samples were incubated with primary antibody(1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.