Immunohistochemistry analysis in formalin fixed paraffin embedded human breast carcinoma using 039128 followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of 039128 for immunohistochemistry.

Fluorescent confocal image of Hela cell using 039128 (1:25). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with 039128 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.