All lanes: 033450 (1:1000) Lane 1: A549 whole cell Lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20ug/lane.

All lanes: 033450 (1:1000) Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SW480 whole cell lysate Lane 5: A549 whole cell Lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 104kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining CD130 in Human skeletal muscle tissue sections by Immunohistochemistry (FFPE) using 033450. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with 033450 (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue using 033450 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of 033450 for immunohistochemistry. Clinical relevance has not been evaluated.