All lanes: 035580 (1:500) Lane 1: CCRF-CEM whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 33kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using 035580 was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA buffer, pH9. 0. Samples were incubated with 035580 (1:100) for 15 minutes at RT. Leica Bond Polymer Refine Detection was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human appendix tissue using 035580 was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA, buffer pH9. 0. Samples were incubated with 035580 (1:100) for 15 minutes at RT. Leica Bond Polymer Refine Detection was used as the secondary antibody.