Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with pervanadate (1 mM; +), using 047591 (left). The phospho-specificity of the antibody was verified by preincubating the antibody with c-Cbl (Tyr700) phosphopeptide (center) or with c-Cbl (Tyr700) nonphosphopeptide prior to incubation with the membrane (right).