Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or treated with λ-phosphatase (+), using 592411 (upper) and S6 Ribosomal Protein (R2031-32D) (lower).

Confocal immunofluorescent analysis of HeLa cells, serum-starved overnight and then treated with Rapamycin (100 nM, 2 hr; left) or FBS (20%, 30 min; right), using 592411 (green). Actin filaments were labeled with DyLight™ 650 Phalloidin (red). Blue = DAPI (fluorescent DNA dye).