Western Blot using I3000-26A (1:500) Lane 1: C2C12 lysate Lane 2 A431 lysate Were resolved by electrophoresis, transferred to PVDF membrane and . Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.

Western Blot using I3000-26A. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.

IKKbeta staining on HeLa cells, pretreated with citrate buffer, pH 6.0. A previous lot of I3000-26A was used at 1ug/ml, using IHC-Select® detection with HRP-DAB. Immunoreactivity is detected as nuclear in tumor cells.

Negative control on HeLa cells, pretreated with citrate buffer, pH 6.0. Antibody diluent incubation, IHC-Select detection with HRP-DAB. No signal was detected.