Western Blot analysis using 042148 (1:2000). Lane 1: mouse lung lysates Lane 2: human liver lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20ug/lane. Secondary: IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 24kD Blocking/Dilution buffer: 5% NFDM/TBST.

Western Blot analysis using 042148 (1:1000). Lane 1: human kidney lysates Lane 2: human liver lysates Lysates/proteins at 20ug/lane. Secondary: IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 24kD Blocking/Dilution buffer: 5% NFDM/TBST

Western Blot analysis of recombinant human SOST protein lysate using 042148 (1:2000). Secondary: IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 24kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using 042148 (1:500) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with 042148 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.