Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing one of two indicated N- and C- terminal fragments of Cas9 (C. jejuni) using 045789 (upper), Myc-Tag (71D10) Rabbit mAb (045696) (middle), and β-Actin (13E5) Rabbit mAb (A0760-41J) (lower). The predicted epitope of Cas9 (C. jejuni) Antibody is a peptide region surrounding Val965 of Cas9 (C. jejuni) protein. As anticipated, Cas9 (C. jejuni) Antibody detects bands corresponding to C-terminal fragment of Cas9 (C. jejuni) and does not cross-react with the N-terminal fragment of the protein.