Western Blot analysis of lysate from human placenta tissue lysate (20ug), using P4071-01C (1:1000). An IgG H&L (HRP) goat anti-rabbit (1:10000) was used as the secondary antibody.

Western Blot analysis of lysate from human placenta tissue (35ug), using P4071-01C (1:1000). A goat anti-rabbit IgG H&L (HRP) (1:5000) was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human small intestine section using P4071-01C (1:100). An IgG (HRP) goat anti-rabbit (1:400) was used as the secondary antibody, followed by DAB staining.

Fluorescent confocal image of HeLa cells stained with P4071-01C. HeLa cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with P4071-01C (1:100, 2hrs at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.