Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using 042220. This demonstrates that 042220 detected the SPECC1 protein (arrow).

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using 042220 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Flow Cytometry analysis of HL-60 cells using 042220 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of U-251MG cells using 042220 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).