R-Spondin 1 Antibody AF3474 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemR-Spondin 1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF3474
NCBI Gene AliasesRspondin, R-spondin, RSPO, CRISTIN3
ApplicationsBioassay,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin
ReactivityHuman,Mouse
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
TargetR-Spondin 1
Conjugate/TagUnconjugated
IsotypeIgG
NCBI Full Gene NameR-spondin 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse R-Spondin 1 (R&D Systems, Catalog # 3474-RS), Ser21-Gly209, Accession # Q9Z132
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant human R-Spondin 1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) R-Spondin 3 and rmR-Spondin 4 is observed.

Sizes Available: 100 ug (also 25 ug)

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Leucine Attenuates Osteoarthritis via mTORC1/LXRα-Mediated Macrophage Reprogramming and Rspo2/β-Catenin Axis SuppressionCARTILAGENovember 7, 2025Ke Zheng, Jiebin Zhang, Wenxiang Liu, Bin Xuat 4 °C with primary antibodies against CD206 (Abcam, Cat# ab64693, UK) or RSPO2 (R&D Systems, Cat# AF3474, USA), followed by HRP-conjugated secondary antibody (Sigma-Aldrich, Cat# A6154, USA) for 1 h at RT.

(2) Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-DependentAnimals : an open access journal from MDPIJuly 7, 2023Zheng M, Liu X, Meng Y, Lin X, Li J, Zhu J, Zhao M, Liu L, Geng T, Gong D, et al.ImageJ V1.8.0.112 software (National Institutes of Health, Bethesda, MD, USA). The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody

(3) Adult mouse fibroblasts retain organ-specific transcriptomic identityeLifeMarch 16, 2022Forte E, Ramialison M, Nim HT, Mara M, Li JY, Cohn R, Daigle SL, Boyd S, Stanley EG, Elefanty AG, et al.OriGene, rabbit polyclonal, 1:50), PAX8 (NBP2-29903, Novus Biological, mouse monoclonal, 1:100), RSPO1 (AF3474, R&D Novus, goat polyclonal, 1:50), and TNT (RC-C2, DSHB 1:200). Cells were stained overnight at 4oC,

(4) RSPOs facilitated HSC activation and promoted hepatic fibrogenesisOncotargetSeptember 27, 2016Yin X, Yi H, Wang L, Wu W, Wu X, Yu L.

(5) R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesisPloS oneJanuary 1, 2015Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G.at 4°C. After quenching, membranes were incubated with anti-RSPO1 (1:1000 dilution, goat polyclonal, AF3474, R&D System, Minneapolis, Minnesota, USA), anti-DKK1 (1:200 dilution, goat polyclonal, sc-30785, Santa Cruz Biotechnology

(6) R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell massDiabetologiaJuly 1, 2011Wong VS, Oh AH, Chassot AA, Chaboissier MC, Brubaker PL.

(7) Human RSPO1/R-spondin1 is expressed during early ovary development and augments β-catenin signalingPloS oneJanuary 28, 2011Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC.antibodies: anti-HA probe (1∶500) (sc-7392, Santa Cruz Biotechnology Inc.) or anti-R-spondin1 (1∶300) (AF3474, R&D Systems). Bound antibodies were detected with enhanced chemiluminescence (ECL kit, Amersham). Apparent

(8) Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in micePloS oneJanuary 1, 2011Saha S, Bhanja P, Kabarriti R, Liu L, Alfieri AA, Guha C.

Be the First to Write a Review!

R-Spondin 1 Antibody

R-Spondin 1 Antibody

R-Spondin 1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (8)

Reviews

R-Spondin 1 Antibody from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map