Western Blot analysis of extracts from human cortex (lane 1), neonatal mouse brain, untreated (lane 2) or phosphatase-treated (lane 3), and fetal rat brain (lane 4), using 583353 (IF preferred) (upper), 136967 Tau (Tau46) Mouse mAb (middle) and β-Actin Rabbit mAb (lower).

Western Blot analysis of extracts from MEF cells (lane 1) and mouse brain (lane 2) using 583353 (IF preferred) (upper), 136967 Tau (Tau46) Mouse mAb (middle) and β-Actin Rabbit mAb (lower).

Confocal Immunofluorescent analysis of Tg2576 mouse brain, untreated (left) or Lambda Protein Phosphatase-treated (right), using 583353 (red) and GFAP Mouse mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Confocal Immunofluorescent analysis of mouse primary neurons using P583353 (green). Blue pseudocolor = Hoescht 33342 (fluorescent DNA dye).