All lanes : 034668 (1:1000) Lane 1: NCI-H1299 whole cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain whole cell lysate Lysates/proteins at 20/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000) Predicted band size: 65kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human brain tissue using 034668 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT, antigen retrieval was by heat mediation with a EDTA buffer , pH9. 0. Samples were incubated with 034668 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human testis tissue using 034668 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT, antigen retrieval was by heat mediation with a EDTA buffer , pH9. 0. Samples were incubated with 034668 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with 034668 (1:25), followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1:100) (red).