Western Blot analysis in MDA-MB453, CEM, NCI-H460, HepG2, K562 cell line lysates (35ug/lane) using 036741. This demonstrates that 036741 detected the HP protein (arrow).

Immunohistochemistry analysis of paraffin embedded human hepatocarcinoma using 036741. Tissue was fixed with formaldehyde at RT.Aantigen retrieval was by heat mediation with a EDTA buffer pH 9.0. Samples were incubated with 036741 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemistry analysis of paraffin embedded human colon tissue using 036741. Tissue was fixed with formaldehyde at RT.Aantigen retrieval was by heat mediation with a EDTA buffer pH 9.0. Samples were incubated with 036741 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using 036741 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 036741 for immunohistochemistry.