Western Blot analysis in PC-3 whole cell lysate (20ug/lane) using 034534 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with 034534. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with 034534 (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.

Flow Cytometric analysis of 293 cells using 034534 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.