Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb 9167L from Cell Signaling Technology

Product Specs
ItemPhospho-Stat1 (Tyr701) (58D6) Rabbit mAb
CompanyCell Signaling Technology
Price $968.00 Inquire
Catalog Number9167L
Quantity300 µl
ApplicationsW, W-S, IP, IHC-P, IF-IC, FC-FP, ChIP, ChIP-seq
ReactivityH, M
TargetStat1 (Tyr701) phosphate
ModificationsStat1 (Tyr701) phosphate
HostRabbit
NCBI Gene AliasesCANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
ClonalityMonoclonal
NCBI Full Gene Namesignal transducer and activator of transcription 1
Research AreaImmunology and Inflammation
IsotypeIgG
Clone58D6
Molecular Weight84, 91

Questions and Answers

Can this antibody be used to study the phosphorylation status of STAT1 in response to TRAIL treatment in breast cancer cells?

Yes, the Phospho-Stat1 (Tyr701) Rabbit mAb has been shown to detect phosphorylated STAT1 levels in response to TRAIL treatment in breast cancer cells, specifically in MCF-7 cells. The phosphorylation of STAT1 was observed as a late event after 24 hours of TRAIL stimulation, indicating its utility in studying interferon signaling pathways in this context ().

Is this antibody effective for detecting phosphorylated STAT1 in pancreatic cancer research?

The Phospho-Stat1 (Tyr701) Rabbit mAb has been utilized in studies involving pancreatic cancer, where it was employed to assess the phosphorylation of STAT1 in response to various treatments, including those that activate the immune system. For instance, it was used to evaluate the effects of the STING pathway activation in apoptosis-resistant pancreatic carcinoma cells ().

What species can this antibody be used on for detecting phosphorylated STAT1?

This antibody is reactive with both human (Homo sapiens) and mouse (Mus musculus) samples, making it suitable for studies involving these species in various experimental setups.

How has this antibody been utilized in the context of interferon signaling?

The Phospho-Stat1 (Tyr701) Rabbit mAb has been cited in research demonstrating its role in sustaining interferon signaling. For example, it was used to show that the deubiquitinase USP2a helps maintain levels of phosphorylated STAT1, which is crucial for effective antiviral signaling mediated by interferons ().

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Pleiotropic effects of BET inhibition broadly boost tumor immunogenicity to CD8+ T cellsOncoimmunologyDecember 31, 2026Jeroen Melief, Lucas Baldran-Groves, Marc-Antoine Gerault, Ying Yu Liang, Sylvya Pasca, Mireia Cruz de Los Santos, Stina Wickström, Tanja Lövgren, Anderson Daniel Ramos, Lars-Gunnar Larsson, et al.primary antibodies overnight at 4 °C: phospho-Jak1 (3331S, Cell Signaling Technology) and phospho-STAT1 (9167S, Cell Signaling Technology). Membranes were probed with HRP- or IRDye-conjugated secondary antibodies

(2) Ceapin-A7 counteracts the protective effects of Lanreotide in endothelial cellsAspects of Molecular MedicineJune 1, 2026Md Matiur Rahman Sarker, Saikat Fakir, Madan Sigdel, Nektarios Barabutis(Radnor, PA). The pMLC2 (95777), pCofilin (3313S), Cofilin (3318S), pP38 (9211S), P38 (9212S), pSTAT1 (9167S), STAT1 (9172S), pERK1/2 (9101S), ERK1/2 (9102S), pSTAT3 (9145S), STAT3 (4904S), BiP (3183S), and Grp94

(3) Interferon stimulatory DNA activates the DNA damage signaling through ATM and DNA-PK sensingJournal of Biological ChemistryMay 1, 2026Samira Kemiha, Lorena Rejón-Franco, Estelle Ghibaudo, Roger J. Eloiflin, Morgane Chemarin, Karim Hawillo, Nadine Laguette, Hervé Técheranti-pTBK1 (5483, Cell Signaling technology), anti-TBK1 (3504, Cell Signaling technology), anti-pSTAT1 (9167, Cell Signaling technology), anti-STAT1 (9172, Cell Signaling technology), anti-pDNA-PK S2056 (ab124918,

(4) HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasionCell Reports MedicineApril 21, 2026Bo Zeng, Yingying Zhou, Heping Wang, Panyin Shu, Ting Pan, Fangfang Liu, Yangyang Li, Ming Yi, Ruirui He, Lingyun Feng, et al.,Cell Signaling Technology,Cat# 3776; RRID: AB_2617123 Phospho-Stat1 (Tyr701) Rabbit mAb,Cell Signaling Technology,Cat# 9167; RRID: AB_561284 Phospho-Stat3 (Tyr705) Rabbit mAb,Cell Signaling Technology,Cat# 9145; RRID: AB_2491009 Mouse

(5) Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic woundsCell Reports MedicineApril 21, 2026Li Lu, Yuan Xiong, Jiewen Liao, Juan Zhou, Guangji Wang, Yating Qin, Shengming Zhang, Yanzhi Zhao, Xiaodan Zhong, Mengwen Wang, et al.,Cell Signaling Technology,Cat#9520; RRID: AB_2193207 Rabbit monoclonal anti-p-STAT1,Cell Signaling Technology,Cat#9167; RRID: AB_561284 Rabbit monoclonal anti-p-STAT3,Cell Signaling Technology,Cat#9145; RRID: AB_2491009 Rabbit

(6) Octreotide Counteracts IFN-γ-Induced Endothelial InflammationJ Biochem Mol ToxicolApril 19, 2026Saikat Fakir, Madan Sigdel, Md Matiur Rahman Sarker, Nektarios Barabutis(3318S), pMLC2 (3674S), MLC2 (8505S), pP38 (9211S), P38 (9212S), pJAK2 (3776S), JAK2 (3230S), pSTAT1 (9167S), STAT1 (9172S), pSTAT3 (9145S), and STAT3 (4904S) antibodies were available from Cell Signaling Technology

(7) Spatio-temporal dynamics of Hendra virus in Australia reveal stable maintenance of diverse viral clades among Pteropus batsNat MicrobiolApril 7, 2026Claude Kwe Yinda, John-Sebastian Eden, Erica T. Prates, Anna Vlot, Sarah van Tol, Sarah L. Anzick, Jianning Wang, Kim Halpin, Benny Borremans, Tamika J. Lunn, et al.1:1,000 pTBK1-172 (Cell Signaling Technology, 5483T), 1:1,000 pSTAT1 – Y701 (Cell Signaling Technology, 9167S), 1:1,000 pSTAT2 – Y690 (Cell Signaling Technology, 88410S), 1:1,000 total STAT1 (Cell Signaling Technology,

(8) The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic disseminationJCI InsightMarch 23, 2026Tarek Taifour, Adéline Massé, Yu Gu, Virginie Sanguin-Gendreau, Dongmei Zuo, Bin Xiao, Emilie Solymoss, Yunyun Shen, Hailey Proud, Sherif Samer Attalla, et al.(1:400; Cell Signaling 70076), CD206 (1:400; Cell Signaling 24595), p-STAT1 (1:400; Cell Signaling 9167), laminin (1:200; Abcam ab11575), collagen IV (1:100; Abcam ab6586), pan-cytokeratin (pan-CK) (1:10;

(9) β-Catenin Stabilization Protects Against Alveolar Hemorrhage Through Amphiregulin and BATF-Mediated Regulatory T CellsJCI InsightMarch 23, 2026Fiona Mason, Hui Xiong, Ali Mobeen, Md Saddam Hossain, Sara Mahmudlu, Rosanne Trevail, Mikyal Mobeen, Li Chen, Sunny Lee, Tuncay Delibasi, et al.Sigma-Aldrich. For signaling analyses, membranes were probed with antibodies against phospho-STAT1 (catalog 9167), total STAT1 (catalog 9172), phospho-STAT3 (catalog 9131), JAK1 (catalog 3332), phospho-JAK3 (catalog

(10) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFront ImmunolMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.Cell Signaling Technology), anti-human STAT1 (14994T, Cell Signaling Technology), anti-human phospho-STAT1 (9167S, Cell Signaling Technology), anti-human CHOP (2895S, Cell Signaling Technology), anti-human β-actin

(11) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFrontiers in ImmunologyMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.Cell Signaling Technology), anti-human STAT1 (14994T, Cell Signaling Technology), anti-human phospho-STAT1 (9167S, Cell Signaling Technology), anti-human CHOP (2895S, Cell Signaling Technology), anti-human β-actin

(12) Keratinocyte-derived Exosomal LKB1 Drives Vitiligo Progression by Activating CD8+ T CellsInflammationMarch 16, 2026Chaoshuai Zhao, Ran Liu, Chuang Gao, Bin Chen, Shu Nie, Xi Tan, Xingyu Mei, Zhouwei Wudilution:1:1000), anti-STAT1 (#14994, Cell Signaling Technology, USA, dilution:1:1000), anti-phospho-STAT1 (#9167, Cell Signaling Technology, USA, dilution: 1:1000). ELISA Assay The ELISA was performed as follows:

(13) Modulation of IRF7-driven transcription as a strategy to control HIV-1 latencyFront ImmunolMarch 16, 2026Ifeanyi Jude Ezeonwumelu, Edurne Garcia-Vidal, Eudald Felip, Sara Cabrero-de las Heras, Bonaventura Clotet, Roger Badia, Ester Ballana, Eva Riveira-Muñoz, Maria NevotPierce); anti-GAPDH (1:2500; ab9485; Abcam); anti-pJAK2 (1:1000; 3774), anti-phosphoSTAT1 (1:1000; 9167), anti-IRF7 (1:1000; 4920), all from Cell Signaling. Blots were immersed in chemiluminescent substrate

(14) TYK2 mediates neuroinflammation in Alzheimer’s disease brains with TDP-43 pathologyNature CommunicationsMarch 14, 2026Laura E. König, Steve Rodriguez, Clemens Hug, Shayda Daneshvari, Alexander Chung, Mark Appleman, Max Tsai, Gary A. Bradshaw, Asli Sahin, Yuyu Song, et al.(cat. # 927-40150; LI-COR) for 1 hour at room temperature. Then, primary antibody (pSTAT1 Y701 (cat. # 9167S; Cell Signaling Technology); beta actin (ACTB, cat. # 3700; Cell Signaling Technology); TYK2 (cat. #ab303500,

(15) Activating mutations in ESR1 contribute to an immunosuppressive breast tumor microenvironment by dampening cytokine secretionJCI InsightMarch 9, 2026Yu Gu, Dongmei Zuo, Qi-Xin Hu, Virginie Sanguin-Gendreau, Alain Pacis, Marie-Christine Guiot, Alexander Chih-Chieh Chang, Tarek Taifour, Chen Ling, Adrian V. Lee, et al.1:50), CD8 (CST, 98941, 1:200), F4/80 (CST, 70076, 1:200), CD206 (CST, 24595, 1:400), p-Stat1 (CST, 9167, 1:200), MHC II (BD, 107601, 1:200), Granzyme B (CST, 44153, 1:200), PD-1 (CST, 84651, 1:200), PD-L1

(16) Orphan Nuclear Receptors expression and function in breast cancer cells: oncogenic action of the NR2F6 receptorCell Communication and SignalingMarch 9, 2026Maria Azzurra Caricasulo, Gabriela Paroni, Adriana Zanetti, Laura Brunelli, Mami Kurosaki, Andrea Vincenzo Cavallaro, Marika Foglia, Gabriella Remoli, Luca Guarrera, Marco Bolis, et al.anti-p65 (NFKB) (Cell Signaling Technology, #8242), anti-STAT1 Tyr701 (Cell Signaling Technology, #9167), anti-IRF1 (Cell Signaling Technology, #8478), anti-RIP1 Ser166 (Cell Signaling Technology, #65746),

(17) Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancerCancer CellMarch 5, 2026Marco Campisi, Tatsuya Osaki, Ian Dryg, Carla Stornante, Jacqueline Wolff, Jason Weirather, Nicholas Weaver, Mubin Tarannum, Ian Gillanders, Alan Bers, et al.(Cell Signaling Technologies) that specifically recognize phosphorylated STAT1 (Y701, clone 58D6, Cat# 9167S), total STAT1 (Cat# 9172S), STING (clone D2P2F, Cat# 13647S) and β-tubulin (clone D2N5G, Cat# 15115S).

(18) Histone demethylase JMJD1A protects mice from enteric bacterial infection by upregulating CCL8 expression to recruit macrophages and CD4+ T cellsPLoS PathogMarch 4, 2026Guifang Lin, Lichun Yang, Shuyan Jiang, Yong Zhang, Ping Luo, Weihua Li, Jianming Xu, Gongpeng Xiong, Chundong Yu, Wenbo Chenanti-JMJD1A (Cat#12835–1-AP, Proteintech), anti-STAT1 (Cat#14994, CST), anti-phosphorylation STAT1 (Cat#9167, CST), anti-IRF-1 (Cat#8478S, CST), and anti-β-actin (Cat #A5441, Sigma-Aldrich). Partly protein band

(19) Thromboxane receptor activation in dendritic cells mitigates sepsis by suppressing S100a8/a9-mediated neutrophil recruitmentSignal Transduct Target TherMarch 3, 2026Ronglu Du, Ting Pan, Yuhan Wang, Yan Fan, Qian Liu, Xixi Tao, Shumin Guo, Danyang Tian, Roger S.-Y. Foo, Keliang Xie, et al.(Cell Signaling Technology, Cat# 9172), rabbit polyclonal anti-pStat1 (Cell Signaling Technology, Cat# 9167), rabbit polyclonal anti-PKCβ (ABclonal, Cat# A21241), rabbit polyclonal anti-pPKCβ (ABclonal, Cat#

(20) Interferon-induced protein IFIT3 as a molecular nexus of neuroinflammation in Alzheimer’s disease and HIV-associated neurocognitive disordersJournal of NeuroinflammationMarch 2, 2026Ranjit Kumar Das, Nirakar Sahoo, Deepa Roy, Lauren Nguyen, Hansapani Rodrigo, Asim K. Duttaroy, Jerel Adam Fields, Upal Roym-IgGκ BP-HRP (catalog #sc-516102) from Santa Cruz Biotechnology (Dallas, TX, USA); STAT1 (catalog #9167S) and HRP-linked anti-rabbit IgG (catalog #7074S) from Cell Signaling Technology (Danvers, MA, USA).

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(1)

4/15/2021Detection of STAT1 Activation in Microglia Lisha JoshiIn our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in ... Read Review
(1)

10/22/2018Useful Antibody for the Detection of Stat1 Phosphorylation Wei Yang SitThe antibody was used in response to cancer cells and how the stat 1 signaling is affected. In summary, this antibody resulted in one band that is sharp and clear.
(1)

12/17/2015Great pSTAT1 Antibody A MOur lab aims to elucidate the role of kinases in several cancers, and we are often performing Western Blots to determine kinase protein and activity levels in various cell lines. For purposes of our... Read Review

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb from Cell Signaling Technology

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Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb from Cell Signaling Technology

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