Flow Cytometric analysis of MDA-MB231 cells using E2265-05 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of Hela cell stained with E2265-05. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with E2265-05 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1h at 37℃). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.