Western Blot analysis in mouse liver cell lysate using 036285. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave using 036285 (1:50). Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.

ISH and IMH demonstrating gremlin mRNA and gremlin protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN using 036285. In comparison with normal renal tissue in which there is no expression of gremlin mRNA (C), proliferating PECs of glomerular crescents show a strong expression of gremlin mRNA by ISH (A); and gremlin protein expression by IMH (B); immune competent infiltrating interstitial cells are also strongly positive for gremlin staining (E); and CTGF was also expressed in these glomerular crescentic cells (F).