Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with 045746 (1:2000) in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.
Western blot analysis of lysates of transfected HEK293 using 045746 in green, dilution 1:2000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. Strong band at ~27kD corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry. The same blot was simultaneously probed with mouse mAb to β-tubulin dilution 1:10,000, in red. The single band ~50kD represents β-tubulin protein expressed in all preparations.
Supplier Page from United States Biological for Pab Rb x Green Fluorescent Protein (GFP) antibody